CH3 H20 CH3-Č-0-CH2-CH2N-CH3 ČH3 Acetylcholine CH3 HO-CH2-CH2N-CH3 + CH3-ç-o-+ H+ ČH3 Choline Acetate
Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.
The concentration of
acetylcholine (a neurotransmitter) in a sample can be determined from the pH changes that
accompany its hydrolysis. When the sample is incubated with the enzyme
acetylcholinesterase, acetylcholine is converted to choline and acetic acid, which
dissociates to yield acetate and a hydrogen ion:
In a typical analysis, 15 mL of an aqueous solution containing an unknown amount of
acetylcholine had a pH of 7.65. When incubated with acetylcholinesterase, the pH of the
solution decreased to 6.87. Assuming there was no buffer in the assay mixture, determine
the number of moles of acetylcholine in the 15 mL sample.
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