Design a radiotracer experiment that would allow you to determine whichproportion of glucose catabolism in a given tissue preparation occursthrough the pentose phosphate pathway and which proportion occursthrough glycolysis and the citric acid cycle. Assume that you can synthesizeglucose labeled with 14C in any desired position or combination of positions.Assume also that you can trap CO, after administration of labeled glucoseand determine its radioactivity.

The ratio will provide the flux rate of carbon through the two pathways.

Answered: Design a radiotracer experiment that…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

In the regulation of glycogen phosphorylase, which of the following statements is true? The inactive T form becomes activated when it gets dephosphorylated. The active R form is inhibited by glucose. Phosphoprotein phosphatase catalyzes the attachment of a phosphate group to a Ser residue in glycogen phosphorylase. The allosteric modulator glucose-6-phosphate promotes the conformation change from inactive T state to active R state.
Name two conditions in which the enzymes involved in the TCA cycle might be inhibited.
Yarrowia lipolytica(Yl) is a non-conventional yeast that diverged from baker’s yeast, Saccharomyces cerevisiae(Sc), early in evolutionary history. The regulation of glycolytic enzymes in Yl differs from that of Sc. The figures below show how PFK activity is affected by fructose-2,6-bisphosphate and phosphoenolpyruvate. Based on these data, which molecule is the major regulator of PFK activity in each species?
An individu with chronic hypoglycemia was suspected of having a defect in one of the enzymes unique to gluconeogenesis. To identify the defective enzyme, tissue samples from a normal liver were compared to samples from the patient's liver biopsy, using a biochemical assay that measures glucose production from glycerol or malate. It was found that incubation with glycerol produced normal amounts of glucose in both the control and biopsied liver samples; however, incubation with malate did not lead to glucose production in the liver biopsy, even though it did lead to glucose production in the control liver sample. Based on these observations, which of the 4 unique gluconeogenesis enzymes is most likely defective in the individual? Consider each enzyme and explain your choice, including why you ruled out enzymes that you did not choose. To answer this question, review how glycerol enters gluconeogenesis (figure 9.48) and how citrate cycle metabolites are transported in and out of the…
In the clinical case scenario provided, match the defective enzyme in Column A with the consequence of this enzyme defect in Column B. IND OAD DH Autopsy results from individual X showed normal levels of glucose and pyruvate, very low levels of NAD+ and very high levels of NADH. Their blood oxygen levels were normal, although the cause of death was given to be a lack of oxygen. Option A B C D Defective enzyme Phosphofructokinase Pyruvate dehydrogenase Cytochrome oxidase Lactate dehydrogenase write your answer here Consequence of this enzyme defect Impaired ATP production Impaired NAD production Impaired respiration Impaired oxygen I L OORD ORD OND DORO G
An investigator briefly incubates an actively respiring bacterial culture with [1-14 C]glucose and isolates the glycolytic and citric acid cycle intermediates. Where is 14 C located in each of the intermediates listed? Consider only the initial incorporation of 14C in the first pass of labeled glucose through the pathways. fructose 1,6-bisphosphate glyceraldehyde 3-phosphate phosphoenolpyruvate acetyl-CoA citrate ☐ ☐ ☐ ☐ ☐ ☐ ☐ a-ketoglutarate oxaloacetate Answer Bank C-4 C-1 C-3 C-2
Glucocorticoids (GC) are stress hormones that modulate glucose homeostasis. One effect is to reduce glucose uptake from the bloodstream into skeletal muscle and adipose tissue, to increase the catabolism of expendable proteins in the skeletal muscle, and to increase gluconeogenesis in the liver. Why are these metabolic adaptations important during stressful situations, like fasting/starvation? Predict how the glucocorticoid cortisol will affect blood urea concentrations.
Can you answer all the parts to this question please List effectors of glycogen phophorylase: A. When blood [glucose] is high. B. When blood [glucose] is low.

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS