Arachidonic acid (mm) 0.295 0.354 0.531 0.885 2.07 Vmax = Km Rate of formation of PGG2 (mM/min) = 14.7 16.5 20.8 26.4 34.2 The kinetic data given in the table are for the reaction catalyzed by a mutant of prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the Vmax and Km of the enzyme. Rate of formation of PGG2 with 10 mg/ml ibuprofen (mm/min) 9.17 10.6 14.1 19.4 28.5 mM/min mM
Prostaglandins are a class of eicosanoids, fatty acid derivatives with a variety of extremely potent actions on vertebrate tissues. They are responsible for producing fever and inflammation and its associated pain. Prostaglandins are derived from the 20-carbon fatty acid arachidonic acid in a reaction catalyzed by the enzyme prostaglandin endoperoxide synthase. This enzyme, a cyclooxygenase, uses oxygen to convert arachidonic acid to PGG2, the immediate precursor of many different prostaglandins.
Attempt 2
Prostaglandins are a class of eicosanoids, fatty acid derivatives with a variety of extremely potent actions on vertebrate tissues. They are responsible for producing fever and inflammation and its associated pain. Prostaglandins are derived from the 20-carbon fatty acid arachidonic acid in a reaction catalyzed by the enzyme prostaglandin endoperoxide synthase. This enzyme, a cyclooxygenase, uses oxygen to convert arachidonic acid to PGG2, the immediate precursor of many different prostaglandins.
Arachidonic acid (mM) Rate of formation of PGG2 (mM/min) Rate of formation of PGG2 with 10 mg/ml ibuprofen (mM/min)
0.295 14.7 9.17
0.354 16.5 10.6
0.531 20.8 14.1
0.885 26.4 19.4
2.07 34.2 28.5
The kinetic data given in the table are for the reaction catalyzed by a mutant of prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the ?max
and ?m
of the enzyme.
?max=
mM/min
?m=
x10y
mM
Trending now
This is a popular solution!
Step by step
Solved in 4 steps with 6 images
