Prostaglandins are one class of the fatty acid derivatives called eicosanoids. Prostaglandins produce fever and inflammation, as well as the pain associated with inflammation. The enzyme prostaglandin endoperoxide synthase, a cyclooxygenase, uses oxygen to convert arachidonic acid to PGG2, the immediate precursor of many different prostaglandins. Ibuprofen inhibits prostaglandin endoperoxide synthase, thereby reducing inflammation and pain. The kinetic data given in the table are for the reaction catalyzed by prostaglandin endoperoxide synthase in the absence and presence of ibuprofen. [??????????? ????][Arachidonic acid] (??)(mM) Rate of formation of PGG2 (?? ???−1)(mM min−1) Rate of formation of PGG2 with 10 ??/??10 mg/mL ibuprofen (?? ???−1)(mM min−1) 0.5 23.5 16.67 1 32.2 25.25 1.5 36.9 30.49 2.5 41.8 37.04 3.5 44.0 38.91 Based on the data, determine the ?max and the ?m of the enzyme
Prostaglandins are one class of the fatty acid derivatives called eicosanoids. Prostaglandins produce fever and inflammation, as well as the pain associated with inflammation. The enzyme prostaglandin endoperoxide synthase, a cyclooxygenase, uses oxygen to convert arachidonic acid to PGG2, the immediate precursor of many different prostaglandins. Ibuprofen inhibits prostaglandin endoperoxide synthase, thereby reducing inflammation and pain. The kinetic data given in the table are for the reaction catalyzed by prostaglandin endoperoxide synthase in the absence and presence of ibuprofen. [??????????? ????][Arachidonic acid] (??)(mM) Rate of formation of PGG2 (?? ???−1)(mM min−1) Rate of formation of PGG2 with 10 ??/??10 mg/mL ibuprofen (?? ???−1)(mM min−1) 0.5 23.5 16.67 1 32.2 25.25 1.5 36.9 30.49 2.5 41.8 37.04 3.5 44.0 38.91 Based on the data, determine the ?max and the ?m of the enzyme
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Prostaglandins are one class of the fatty acid derivatives called eicosanoids. Prostaglandins produce fever and inflammation, as well as the pain associated with inflammation. The enzyme prostaglandin endoperoxide synthase, a cyclooxygenase, uses oxygen to convert arachidonic acid to PGG2, the immediate precursor of many different prostaglandins.
Ibuprofen inhibits prostaglandin endoperoxide synthase, thereby reducing inflammation and pain. The kinetic data given in the table are for the reaction catalyzed by prostaglandin endoperoxide synthase in the absence and presence of ibuprofen.
| [??????????? ????][Arachidonic acid] (??)(mM) |
Rate of formation of PGG2 (?? ???−1)(mM min−1) |
Rate of formation of PGG2 with 10 ??/??10 mg/mL ibuprofen (?? ???−1)(mM min−1) |
|---|---|---|
| 0.5 | 23.5 | 16.67 |
| 1 | 32.2 | 25.25 |
| 1.5 | 36.9 | 30.49 |
| 2.5 | 41.8 | 37.04 |
| 3.5 | 44.0 | 38.91 |
Based on the data, determine the ?max and the ?m of the enzyme.
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