Answered: Describe the different types of GC…

Describe the different types of GC instrumentation and column types available, and provide some of the pro’s and con’s of each particular type for pharmaceutical and forensic analysis.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter28: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 28.3QAP

See similar textbooks

Related questions

Q: A researcher was attempting to quantify the amount of dichlorodiphenyltrichloroethane (DDT) in…

A: To determine the response factor and the amount of DDT in the sample as milligrams of DDT per gram…

Q: What are the advantages and disadvantages of using external standards in instrumental analysis?

A: • External standards are the most common form of standardizations - external standards are prepared…

Q: 4. Determine a procedure to accurately create a 1.5x10 mg Fe/mL solution using a 0.0500 mg Fe/mL…

A: Given:The initial concentration of the standard solution = The target concentration of the final…

Q: A banned pesticide, DDT, is analyzed through gas chromatography. A DDT standard is diluted…

A: In analytical chemistry a calibration curve or standard curve, is plotted to determine the…

Q: Consider a separation performed on a 30.0 mm long open tubular column with a 0.50 mm diameter and a…

A: Length of column(L) = 30.0 mmDiameter of column(d) = 0.50 mmThickness(t) = 2.0 micrometerRetention…

Q: calculate the dilution required to analyse the concentration of paracetamol in a commercially…

A: Molar extinction coefficient (ε) of Acetaminophen at 250 nm = 14 mM-1cm-1…

Q: A second spectrophotometric method for the quantitative analysis of Pb2+ in blood yields an Ssamp of…

A: The value of sensitivity of the method for the analyte (kA) is calculated using sample signal…

Q: what is the purpose of BSC Level 1 & 2, proper layout, performance, and its application in biosafety…

A: Biosafety labs deal with infectious diseases.when workings with infectious materials, proper safety…

Q: One method for the quantitative determination of the concentration of constituents in a sample…

A: Given: The relative areas are 16.4, 45.2 and 30.2. The relative detector responses are 0.60,…

Q: Describe the primary and secondary criteria for having achieved V ̇O2 max.

A: V̇O2 max (also maximal oxygen consumption, maximal oxygen uptake or maximal aerobic capacity) is…

Q: After establishing the response factor of the instrument, the researcher collected 14.58 g of…

A: Response factor (RF) = 0.0170 Concentration of chloroform calculation: Amount of chloroform taken…

Q: e used to determine whether the concentrations of haloacetates in the treated water demonstrate…

A: Here, the DBPR - two levels. The level 1 of DBPR new standards and a treatment technique of enhanced…

Q: You are developing a procedure for determining traces of copper materials using a wet digestion…

Q: A 0.0200 gram blood sample was decomposed by a microwave digestion technique followed by dilution to…

A: A 0.0200 gram blood sample containing Pb was estimated with the help of lead complexing agentby…

Q: 1 mL was taken from the sample filtrate and mixed with 13 mL pure water and 4 mL sulfomolybdic acid…

Q: urses/ 2951 2. A linear standard curve of KMnO4 is prepared from a set of standard solutions by…

A: The objective of this question is to determine the slope of the standard curve of KMnO4 and use it…

Q: 5-3. Question 5-2 refers to separating a mixture of two high boiling liquids by gas chromatography.…

A: We have find out the answers.

Q: The pH of water samples taken from Iligan Bay was determined. Calculate the pooled estimate of the…

A: The formula of standard deviation is shown below: σ =∑n=1ixi-x-2n-1 where; x- = mean n = number of…

Q: You prepare the following MeOH standards from a 2000 ppm stock MeOH solution: Calibration Blank, 50…

A: We have to give the , formula & R2 value of given data and determine the linear working range…

Q: A solution of volume 1 mL, containing an equal parts mixture (by weight) of aspirin and paracetamol…

A: (i) The 2.0 min peak in the unknown sample can be confirmed as paracetamol if it exhibits the same…

Q: A solution containing an equal parts (by weight) mixture of two liquids A and B dissolved in ethanol…

A: Given: retention time, baseline width, integration of compounds A and B and the retention time of…

Q: If some of the analytes were found to be out of range after doing the Quality Control and the…

A: Quality control is designed to detect ,check the actual quantity of analyte before patient's use.…

Q: In a GC run, dodecane was used as test solute for a 30 m x 250 ?m i.d. DB-5 column. The dodecane…

A: Theoritical plate in chromatography is a key parameter of chromatography. It is a index of coloumn…

Q: (5) If 200 g of polymer A, 300 g of polymer B, 500 g polymer C, and 100 g of polymer D are mixed,…

A: Molecular mass of polymer is a most important parameter and directly related to the physical…

Q: What is the actual or real-life application of precipitation gravimetric analysis? Include the…

A: Precipitation gravimetry is a scientific method that utilizes a precipitation response to isolate…

Q: Anionic surfactants in wastewater are measured spectrophotometrically as methylene blue active…

A: Given: Limit of surfactant content in Philippine class B water is 300 ppb

Q: Bradford assay was used to determine bovine serum albumin (BSA) concentrations. The method works by…

A: The above question pertains to the Bradford assay, a widely used method for determining protein…

Q: A sample of enzyme involved in alcohol metabolism, extracted and purified extensively from yeast…

A: Given:

Q: A standard sample contains 15.0 mM of analyte and 25.0 mM of internal standard. The analysis of the…

A: Internal standard method: In analytical chemistry addition of chemical substance to a sample is in…

Q: With the following information for a GC analysis using an internal standard, calculate the…

Q: Briefly explain method validation in analytical chemistry? Discuss typical validation…

A: Answer - Analytical chemistry - Analytical chemistry is the science of obtaining, processing, and…

Q: The student was supposed to measure the pH of a 0.1 M hydrochloric acid solution. For this purpose,…

A: Concentration of HCl = 0.1 M

Q: method as follows: punty of an ibuprofen sample was determined by a reversed phase HPLC Y A…

A: Equation for the best-fit line of the calibration curve is: y=26820x-1323.8

Q: der the peaks for pentafluorobenzene and benzene in the gas chromatogram shown here. The elution…

A: Given data: Elution time for unretained solute = 1.06 min Length of column = 30 m Diameter of column…

Q: The concentration of acetylsalicylic acid in an antipyretic tablet was determined by HPLC using a…

A: A numerical problem based on quantitative analysis, which is to be accomplished.

Q: The researcher extracts a sample of well water and prepares it for TXRF analysis with a 10.00 mg/L V…

A: As per the given data, TXRF = 10.00 mg/L Calibration coefficient = 69297.81

Q: 3. This is the standard procedure for determination of nicotine in urine. A 1.00mL sample was placed…

A: A question based on tools in analytical chemistry that is to be accomplished.

Question

Describe the different types of GC instrumentation and column types available, and provide some of the pro’s and con’s of each particular type for pharmaceutical and forensic analysis.

Scientific analysis of crime scene evidence to determine the events of a crime. Forensic science includes analyzing biological evidence, such as DNA fingerprinting, and non-biological evidence, such as computer forensics.

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1: Introduction

VIEW

Step 2: Working of GC technique

VIEW

Step 3: Type of GC

VIEW

Step 4: Choice of solvent

VIEW

Solution

VIEW

Step by step

Solved in 5 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

(i) Calculate the capacity factors (k') for both compounds A and B and the selectivity factor (a) between them, commenting on the values. (ii) Calculate the resolution between A and B and comment on it. (iii) Using the peak for A, calculate the number of theoretical plates for the GC column and comment on the quality of the column.
2 what is the purpose of BSC Level 1 & 2, proper layout, performance, and its application in biosafety assessment
A solution of volume 1 mL, containing an equal parts mixture (by weight) of aspirin and paracetamol (acetaminophen), was analysed by HPLC, displaying peaks at retention times 1.5 mins (area integration 1500) and 2.0 mins (area integration 2500). An unknown solution, also volume 1 mL, was analysed under the same conditions and, among a number of other peaks, there was one at 2.0 mins (area integration 4000). (i) How can the 2.0 min peak in the unknown sample be confirmed as paracetamol? (ii) In a subsequent analysis, 5.0 mg of aspirin was dissolved in the unknown solution and an extra peak appeared in the HPLC at 1.5 mins (area integration 2000). Assuming the 2.0 min peak confirmed as paracetamol, determine its concentration (g L-¹) in the unknown sample and estimate the error in the determination.
Please answer Questions 5-3, 5-4, and 5-5 (GC Gas Chromatography) 5-2. A sample mixture of ethyl benzoate (bp 212 Celsius) and dodecane (bp 216.2 Celsius) is injected on two GC columns.Column A has DC710 silicone oil as the stationary phase, and column B uses polyethylene glycol as the stationaryphase. Which substance would be certain to elute first from column A and would the same material be expected toelute first from column B? Which column, A or B, would be expected to give the better separation of these two substances? 5-3. Question 5-2 refers to separating a mixture of two high boiling liquids by gas chromatography. These materials havesimilar boiling points. List several GC variables and conditions that would make it easier to separate thesesubstances by gas chromatography. 5-4. Capillary GC columns have better resolution than packed columns even though the enormous surface area provided by the packing material is absent in capillary columns. Why? 5-5. Preparative GC requires…
A standard sample contains 15.0 mM of analyte and 25.0 mM of internal standard. The analysis of the standard sample gives peak areas for the analyte and internal standard of 1.782 and 3.539, respectively, in a chromatography analysis. Then sufficient internal standard is added to a sample to make its concentration 10.0 mM. The analysis of this new sample yields peak areas for the analyte and internal standard of 0.894 and 2.981, respectively. Name the calibration method and report the analyte's concentration in the new sample. (e)
A second spectrophotometric method for the quantitative analysis of Pb2+ in blood yields an Ssamp of 0.712 for a 5.00 mL sample of blood. After spiking the blood sample with 5.00 μL of a 1560 ppb Pb2+ external standard, an Sspike of 1.546 is measured. What is the concentration of Pb2+ in the original sample of blood?
6
The researcher extracts a sample of well water and prepares it for TXRF analysis with a 10.00 mg/L V internal standard, like the calibration standards. The result produces SCa = 69297.81 and SV = 13481.2.Determine the concentration of Ca.
3. This is the standard procedure for determination of nicotine in urine. A 1.00mL sample was placed in a 12mL vial 5-aminoquinoline was added as the internal standard (5 ug). The sample was monitored using mass spectrometry at m/z values for nicotine (84) and the internal standard (144). Given the following data determine the concentration of the two unknown samples Nicotine (ug/L) Area Area 12 0.056 0.059 51 0.402 0.391 102 0.684 0.669 157 1.011 205 Unknown 1 Unknown 2 1.278 0.51 1.18 1.063 1.355 0.53 1.32
Acetaminophen or paracetamol, the active ingredient in many pain killers absorbs in the UV range with a λmax at 250 nm in neutral solutions (methanol usually). Its extinction coefficient is 14 mM-1cm-1. Each commercially available tablet (Tylenol, Panadol or Herron) contains 500 mg paracetamol. Its molecular weight is 151.1. Using this information, calculate the dilution required to analyse the concentration of paracetamol in a commercially available paracetamol tablet. Start by dissolving the tablet in 100 mL methanol. Show working out.
Briefly explain method validation in analytical chemistry? Discuss typical validation characteristics which could be considered. Name some international organisations offering guidelines on method validation
Pesticide residues were found in a lot of oranges; the compound identified was the epoxide of heptachlor (EH). To quantify the amount of this pesticide in oranges, 20 grams were taken from fruit peels and mixed with methylene chloride to extract it. The concentration of heptachlor epoxide in the sample was determined by gas chromatography coupled to a detector mass (GC-MS); and calibration was performed using anthracene (A) as internal standard. For In this analysis, the anthracene had a concentration of 350 ppm and the same volume was added to each standard. The table below shows the concentrations of the standard and the ratio of the areas.and the sample is: 0.1080.Determine the concentration of heptachlor epoxide in the sample.