Can you please check my answer and make sure it is correct. Question: List the ingredients of master mix, and state the purpose of each ingredient. Answer: Taq DNA polymerase This enzyme synthesizes the complementary strand of the DNA template after attaching to the primer. This means that it adds on free nucleotides to the existing strand, but helps speed up the covalent bonding between these newly added nucleotides. This enzyme is also thermally stable meaning that it can withstand the hot temperatures needed for PCR to occur. This hot temperature is needed for the denaturation step when the double stranded DNA has to be unwound and separated into two strands. Individual building blocks of DNA (either free nucleotides A, T, C, and G or dNTP’s) These nucleotides are needed to build the complementary strand of DNA A special buffer to maintain the optimal pH, salts, and MgCl2 These buffers help maintain a good pH that doesn’t become too acidic or basic for Taq DNA polymerase to operate. DNA itself is pH sensitive which means that its bonds can be disturbed if this buffer isn’t used Salts can help stabilize primer-template binding during the annealing process of PCR to help Taq DNA polymerase start extension MgCl2 is added to the reaction because it essentially acts as a catalyst for Taq polymerase. When this is added into the reaction, it helps the reaction proceed more quickly (however you don’t want too much present because then the Taq polymerase will work too quickly and will be prone to making mistakes during the synthesizing process) Cofactors (salts and magnesium ions) are used so that the conditions are optimal for Taq polymerase to perform These cofactors are used to stabilize the primer-template binding, and catalyze the process of Taq polymerase synthesizing the DNA strand
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
Can you please check my answer and make sure it is correct.
Question: List the ingredients of master mix, and state the purpose of each ingredient.
Answer:
- Taq DNA polymerase
- This enzyme synthesizes the complementary strand of the DNA template after attaching to the primer. This means that it adds on free
nucleotides to the existing strand, but helps speed up the covalent bonding between these newly added nucleotides. This enzyme is also thermally stable meaning that it can withstand the hot temperatures needed for PCR to occur. This hot temperature is needed for the denaturation step when the double stranded DNA has to be unwound and separated into two strands. - Individual building blocks of DNA (either free nucleotides A, T, C, and G or dNTP’s)
- These nucleotides are needed to build the complementary strand of DNA
- A special buffer to maintain the optimal pH, salts, and MgCl2
- These buffers help maintain a good pH that doesn’t become too acidic or basic for Taq DNA polymerase to operate. DNA itself is pH sensitive which means that its bonds can be disturbed if this buffer isn’t used
- Salts can help stabilize primer-template binding during the annealing process of PCR to help Taq DNA polymerase start extension
- MgCl2 is added to the reaction because it essentially acts as a catalyst for Taq polymerase. When this is added into the reaction, it helps the reaction proceed more quickly (however you don’t want too much present because then the Taq polymerase will work too quickly and will be prone to making mistakes during the synthesizing process)
- Cofactors (salts and magnesium ions) are used so that the conditions are optimal for Taq polymerase to perform
- These cofactors are used to stabilize the primer-template binding, and catalyze the process of Taq polymerase synthesizing the DNA strand
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
