Calculate the delta G of proton transport through the mitochondrial inner membrane. Is this a spontaneous or nonspontaneous process?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Calculate the delta G of proton transport through the mitochondrial inner membrane. Is this a spontaneous or nonspontaneous process?

Objective: Perform calculations about the free energy change and electric potential
across various membranes, illustrating differences between them.
1. Recall the membrane proton-transport free energy equation:
AG = 2.3 RT [pH(side 1) - pH (side 2)] + Z FAY
=
where Z = the charge on the proton (+1), F = Faraday's constant = 96485 C/mol and R
gas constant = 8.314 J/K mol.
For the inner membrane of a mitochondrion, AY = + 0.168 V; use T = 310K (37°C) and
pH(matrix) - pH (intermembrane space) = 0.75.
Transcribed Image Text:Objective: Perform calculations about the free energy change and electric potential across various membranes, illustrating differences between them. 1. Recall the membrane proton-transport free energy equation: AG = 2.3 RT [pH(side 1) - pH (side 2)] + Z FAY = where Z = the charge on the proton (+1), F = Faraday's constant = 96485 C/mol and R gas constant = 8.314 J/K mol. For the inner membrane of a mitochondrion, AY = + 0.168 V; use T = 310K (37°C) and pH(matrix) - pH (intermembrane space) = 0.75.
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