You are stumped for a while, because you really don't want to spend all day running columns, and then having tomeasure the column fractions with UV-VIS. Your colleague however tells you that it is possible to use thespectrum of the mixture to determine the concentration of cach vitamin in the mixture.(c) Briefly explain why it is possible to use the spectrum of the mixture to determine the concentration of eachvitamin in the mixture.Now that you realise that it is possible, you get some information about the molar absorptivities of cach vitaminat cach Amax, and list it in Table 1.Table 1. The molar absorptivities of Vitamins B1 and B6 measured in pure samples of each.E (M' cm')a (nm)Vitamin B1Vitamin B64235075240112802851504(d)(1) Use the information in Table 1 and the absorbance of the sample mixture at the relevant wavelengths(Figure 1) to determine the concentration of Vitamin B1 in the diluted, prepared sample.(d)(i) Determine the mass of Vitamin B1 in one of the vitamin tablets. (MW Vitamin B1: 300.81)(e) Sketch a diagram of the double-beam scanning spectrophotometer that you would have used to generatethe spectra in Figure 1.

Beer Lamberts Law states that the Absorbance (A) is equal to the products of molar absorptivity…

Answered: (c) Briefly explain why it is possible…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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(c) Briefly explain why it is possible to use the spectrum of the mixture to determine the concentration of cach vitamin in the mixture.