Using G-25 Sephadex beads (Fractionation range 1000-5000) KD, a sample of Vitamin B12 (MW ~1500 KD) will be: Fully excluded Partially included Fully included the activity of lysozyme was determined by looking at the increase in light scattering per unit time increase in absorbance per unit time. decrease in absorbance per unit time. increase in concentration per unit time.
Q: You are studying the rate of transcription of a particular eukaryotic gene. When the DNA located sev...
A: Transcription is copy of genetic information from DNA to RNA. Eukaryotic transcription is more compl...
Q: Ever eat a maggot? Guess again! The FDA publishes a list, the Food Defect Action Levels list, which ...
A: Given Values: 20 maggots are allowed in 100 grams of the mushroom. The average size of the batch = ...
Q: What is experiment of Thermodynamics of Small Oligomeric Duplex DNA Denaturation.
A: Double-stranded DNA is the most common kind of DNA molecule found in nature. The strands can be unwo...
Q: Draw the structure of the wax composed of stearic acid and a straight-chained alcohol with 30 carbon...
A: Waxes are simple lipids composed of fatty acids (saturated and unsaturated) which are esterified to ...
Q: Unfolding of a protein can be termed as a. Reduction b. Oxidation c. Denaturation d. Renaturation
A: Proteins have different levels of structural organization. They are, Primary structure Secondary st...
Q: Identify the following statements whether correct or incorrect. * CORRECT INCORRECT cellulose are us...
A: Carbohydrates are biological molecules made of carbon, hydrogen, and oxygen.
Q: Enumerate the five classification of lipids based on function.
A: Lipid, any of a wide range of organic molecules that do not interact significantly with water, such ...
Q: What are the different mechanism of disease production?
A: The disease production mechanism identifies the likely source or causes of a problem/disorder, as we...
Q: Describe monosaccharide, disaccharide, oligosaccharide, and polysaccharide in terms of the number of...
A: Biomolecules refer to carbon- based organic compound that are produced by a living organism. These c...
Q: For beta-oxidation of an 18:1 oleate mono-unsaturated fatty acid: - How many Acetyl CoA produced? - ...
A: Fatty acids are molecules with hydrocarbon chain and carboxylic acid group. In the absence of glucos...
Q: Esterase hydrolyzes ester bonds. For simplicity, only the amino acids whose side-chains interact wit...
A: Esterase- group of enzymes which hydrolyses the ester bond from the substrate like acetylcholine, fa...
Q: What would happen to the functionality of cholic acid if the 3 hydroxy groups were removed? A) Ther...
A: Fats are water insoluble amphipathic molecules. More than 90% of the structure of a fat is hydrophob...
Q: Biosynthesis of leucine involves conversion of 1-isopropylmalate to 2-isopropylmalate (see above). T...
A: 2-isopropylmalate synthase is a enzyme which catalyzes below chemical reaction with three substrate...
Q: What would be the consequence for actin filament assembly/disassembly if a mutation prevented actin’...
A: The kinetics of filament formation are modulated by ATP-binding on actin subunits, with ATP-binding ...
Q: A catalase is an antioxidant enzyme that requires ferric ion for catalysis. Under conditions of iron...
A: - Option A- A prosthetic group is a non-amino acid component that is closely connected to the apopro...
Q: Below are the structures of three phosphate-containing molecules. Which one has the lowest Phosphory...
A: Phosphate group is containing one phosphate group atom attached to four oxygen molecule.Various mole...
Q: 3. In Drosophila, an X-linked recessive mutation, scalloped (sd) causes irregular wing margins. Diag...
A: A) a scalloped female is crossed with a normal male: - Xsd Xsd × X Y Xsd Xsd X Xsd X Xsd X ...
Q: Typically, opiates have the ability to a. cause intense hallucinations. b. suppress the sensation of...
A: The correct answer is b. suppress the sensation of pain A drug that is used to relieve pain or induc...
Q: Which two enzymes produce reducing equivalents without a loss of carbon in the TCA cycle? O Aconitas...
A: Question 19: Two enzyme that produce reducing equivalent without the loss of carbon in TCA cycle are...
Q: What chemical test could be used to differentiate a protein from an amino acid? Explain briefl
A: Introduction: Amino acids are biological molecules that contain an amine and a carboxylic group and ...
Q: Explanations are not needed. Direct answers are enough. II. True or False a. An effort is usuall...
A: The protein molecules after their folding have exposed patches of charged amino acids on their surfa...
Q: In the DNA extraction. What is the role of alcohol in the DNA extraction process?
A: DNA extraction involves collecting all the DNA from a cell. For that we first prepare the cells from...
Q: Which of the following chromatographic techniques has an attached ligand on the stationary phase? A...
A: Chromatography is a laboratory technique that is used to separate components, or solutes, of a mixtu...
Q: Compare exotoxin from endotoxin based on the following features
A: Toxins are dangerous substances produced by live cells or organisms; synthetic toxicants made by art...
Q: Disulfide bridges are limited in that only one particular amino acid can form these types of interac...
A: Amino acids are building blocks of proteins and alpha carbon of amino acids consist of carboxylic gr...
Q: Three cell membrane samples: M, C, and S were fluorescently labeled and were subsequently photobleac...
A: Fluorescent Recovery After Photobleaching (FRAP) is a technique used to test the mobility (fluidity...
Q: The initial pH of a 0.2M arginine solution is 14. a. What is the predominant structure of arginine i...
A: Arginine is a basic amino acid and it contains a guanidino group in its side-chain. Guanidino group ...
Q: What is the process and principle behind isoelectric precipitation?
A: Isoelectric precipitation is a type of isoelectric precipitation that occurs when: The pH of a solut...
Q: The enzyme is considered to be alan* COO COO H-C-OH Phosphoglyceromutase H-C-0- ČHO-P ČH,OH 3-Phosph...
A: Enzymes are classified as oxidoreductases, transferases, hydrolases, lyases, isomerases, and ligases...
Q: Drawn below is a schematic of a transmembrane protein. AN Extracellular Cell membrane Cytosolic side...
A: Introduction: A membrane protein is a protein molecule that is connected or linked with the membran...
Q: Match the outcomes on the left with the laboratory steps on the right. ___ Harvest the cells ...
A: Introduction: DNA isolation is a process of purification of DNA from samples using a combination of ...
Q: correct or incorrect. CORRECT INCORRECT Proteins in a primary structure consist of a simple polypept...
A: The base level of the protein hierarchy is the primary structure, which is the specific linear seque...
Q: Using a semi-permeable membrane, dialysis allows the removal of salt ions prior to chromatography. T...
A: Dialysis is a separation process that uses selective and passive diffusion via a semi-permeable memb...
Q: If you have to prepare 4 nM solution of human IgG ( immunoglobuline) which has molecular mass of 150...
A: The number of moles of a solute present in one liter of the solution is called the molarity of that ...
Q: Cleavage of a phosphate bond is needed for de-polymerization of which mature polymer? Neither microf...
A: The eukaryotic cytoskeleton is composed of microfilaments, microtubules, and intermediate filaments....
Q: Which of the followingdescribe superior properties of enzymes (biological catalysts) over traditiona...
A: Enzymes are biocatalysts that are protein in nature. They Enzymes present a the particular substrate...
Q: The configuration of the given structure below is: * но-сн, о OH но CH2-OH он O alpha-L O alpha-D be...
A: The isomeric units of monosaccharides that differ only in their configuration at reference carbon ar...
Q: Propose a reasonable biosynthesis for Compound 14 starting from Acetyl CoA, SAM, S- alanine, S-pheny...
A: Here compound 14 is synthesized from L-Phenylalanine in multistep process. The structure of all the ...
Q: What are the other methods of detection for blood glucose determination? Explain the principle invol...
A: There are different methods for estimating the levels of glucose in the blood. The different methods...
Q: There are two types of nucleic acids-DNA and RNA, both are composed of nucleotide subunits. Complete...
A: The nucleic acid polymer has nucleotide as its monomeric unit. synthesis of nucleotides is a...
Q: Giveee a sufficient biosynthesis for this compound starting from acetyl CoA, S-alanine, S- adenosyl ...
A: Here compound 14 is synthesized from L-Phenylalanine, SAM, L-alanine, and Acetyl-CoA in multistep p...
Q: The enzyme is considered to be alan * COO Lactate COO dehydrogenase HO-C-H + NAD 0 + N
A: LDH, or lactic acid dehydrogenase, is an enzyme that is a type of protein. LDH is critical in the pr...
Q: Assessing the efficacy of glucosamine and chondroitin sulfate for arthritis pain Treatment with...
A: Many patients with arthritis, particularly osteoarthritis, utilise vitamins in their diet to relieve...
Q: Given the following reaction, identify the class and subclass of the enzyme involved. CH2OH C=O H-C-...
A: Enzymes catalyse the reaction either by making or breaking the bond. They might form double bonds or...
Q: Compare and contrast antibiotic and antiviral treatments. Analyze how different antiviral therapies ...
A: Antibiotics are the group of antimicrobial substances which are used to inhibit or to destroy any mi...
Q: The interior core of globular proteins is typically composed of non-polar amino acid residues becaus...
A: Proteins are made up of polypeptide chains of amino acids. The amino acids arrange themselves to for...
Q: A group of BS Biology students were tasked to design and fabricate different synthetic cell membrane...
A: Several factors determine the fluidity of the cell membrane membrane like membrane lipid chemistry, ...
Q: The configuration of the given structure below is: * CH,OH он ÓH O alpha-L O alpha-D O beta -L O bet...
A: The carbohydrates can be represented by two types of configurations: L-isomer and D-isomer. When the...
Q: 1. Given the MRNA: 5'AUGCAUGACGAUCUCGUCGCG...3' a. Use the genetic code to predict the amino acid se...
A: 1. The messenger RNA (mRNA) is produced by the process of transcription from a double-stranded DNA s...
Q: III. True or False a. Large proteins travel/migrate faster in both gel filtration chromatography a...
A: Different techniques used in the separation and purification of the biomolecules are, Chromatography...
Using G-25 Sephadex beads (Fractionation range 1000-5000) KD, a sample of Vitamin B12 (MW ~1500 KD) will be:
Fully excluded |
||
Partially included |
||
Fully included |
the activity of lysozyme was determined by looking at the
increase in light scattering per unit time |
||
increase in absorbance per unit time. |
||
decrease in absorbance per unit time. |
||
increase in concentration per unit time. |
Step by step
Solved in 4 steps
- Using G-25 Sephadex beads (Fractionation range 1000-5000) KD, a sample of Vitamin B12 (MW ~1500 KD) will be: Partially included Fully excluded Fully includedin food microbiology, how do you compute for concentration (M) and absorbance (A) of peroxidase activity on hydrogen peroxide at different pH values? (wavelength used = 415nm). can you please explain the calculations thank you if given: Temperature = 75 C Molar absorptivity coefficient = 10.5 Path length (cm) = 2 I = transmitted light = 0.45Table 1 - Comparison of the effect of catechol concentration on the amount of product formed. Absorbance Potato extract Absorbance 0 mins after 30mins (2nd reading) (mL) 1st reading 1 Tube # la blank 2a 3a 4a 1 1 1 dH₂O Catechol (mL) (mL) 7 5 3 1 0 2 4 6 0.00 0.060 0.033 0-05-2 Q4) Give 2 reasons for adding dH₂O to these tubes in Table 1? Time for reading: 3:21 -0.11 Absorbance: Time for reading: 3.36 Q5) Tube la serves as a control, but why is this control needed? Absorbance: 0.197 Time for reading: 3.37 Based on the data from Table 1 answer these questions: Q1) What is the name of the enzyme found in potato extract? Answer: catechol Q2) What is the substrate? Answer: THO Q3) Name of product of this enzyme catalyzed reaction? Answer: Absorbance: 0.152 Time for reading: 3:39 Absorbance: . 166 ness Catechol Benzoquinone Subtract 1st from 2nd reading -0.01 0-137 0.11.19 0.119 Q6) Notice that your 1st absorbance reading in tubes 2a-4a are quite similar but it then becomes very different…
- Given this, if you used 6g of vitamin Z powder to make 20 ml of solution, what is the % concentration of this solution? (I gave the image since I don't know if that info is needed to solve this question.)It also gives a follow-up, if you can help here too: You work in a lab as a summer student. One of your tasks is to make sure that there is enough cell culture medium containing antibiotics to grow bacteria. One day you realize that there is only 5 ml of 10% Antibiotic stock solution in the freezer. You decide to use it all to prepare the working culture medium with 0.01% antibiotic. In the lab there is plenty of growth medium without antibiotics. (Note: dilution in medium is like dilution in water). You remember the equation to make dilutions of stock solutions. You usually use this formula to calculate the required volume of a stock solution, but you realize it can apply here as well, even though the unknown is the final volume. So, you make that dilution. Given that each bacterial…The reults for the macroscopic part: 0.30M glycerin – solution was translucent (could see text behind the test tube) 0.15M NaCl – solution was opaque (could not see text behind the test tube) 0.30M NaCl – solution was opaque (could not see text behind the test tube) 0.15M glucose – solution was translucent (could see text behind the test tube) 0.30M glucose – solution was opaque (could not see text behind the test tube) 0.30M Urea – solution was translucent (could see text behind the test tube) Results for microscopic part: 0.30M glycerin – no cells present 0.15M NaCl – normal sized cells 0.30M NaCl – crenated (shrunken and star-shaped) cells 0.15M glucose – no cells present 0.30M glucose – normal sized cells 0.30M Urea – no cells present Determine the osmolarity (hypoosmotic, isosmotic, or hyperosmotic) and tonicity (hypotonic, isotonic, hypertonic) of the following solutions.In which solutions did the osmolarity NOT match the tonicity? For those solutions, why did the osmolarity…the 10 mg/mL sample of lysozyme shows a lower activity than 2mg/mL lysozyme sample (refer to the graph shown below). It is potentially due to Inefficiency of lysozyme at high concentrations. Highest concentrated lysozymes do have lowest activity. Loss of data due to user inefficiency. Loss of data due to spectrophotometer inefficiency.
- Standard curves You are setting up a standard curve for lysozyme. You have a stock vial containing 2 mg/ml of lysozyme and you have 2 ml in the vial. You will want to have a minimum of 1 ml of each concentration for the spectrophotometer. How would you proceed? (Saline is added to make the various concentrations). Concentration Am't stock lysozyme or am't of a previous dilution (note which) Amount saline Total volume wanted 1.00 mg/ml 0.75 mg/ml 0.50 mg/ml 0.375 mg/ml100ml of LB media with 25 μg/ml of Amp and 100 μg/ml of Kan final concentration. You have 100ml of LB provided and Amp and Kan stocks at 100 mg/ml and 50 mg/ml provided. Determine how much of each antibiotic stock solution you need to add to 100ml of LB to reach desired antibiotic concentration.The Bradford reagent gives a linear response only from 0.1 mg/mL to 1.4 mg/mL of protein concentration. Would we be able to use an "absorbance vs. concentration" line graph for samples outside of the range indicated? Justify your response.
- Infuse heparin at 1,200 units per hour from a solution containing 40,000 units of heparin in 500 mL D5W. How many mL/hr will deliver the ordered dosage?Many heavy metals are used in antiseptics. Explain the oligodynamic effect and provide a clinical example of the use of heavy metals.Example of a Protein Purification Scheme: Purification of the Enzyme Xanthine Dehydrogenase from a Fungus Volume Total Total Specific Percent Fraction (mL) Protein (mg) Activity Activity Recovery 1. Crude extract 2. Salt precipitate 3. Ion-exchange chromatography |4. Molecular-sieve chromatography 5. Immunoaffinity chromatography 3,800 22,800 2,460 0.108 100 165 2,800 1,190 0.425 48 65 100 720 7.2 29 40 14.5 23 1.8 275 152.108 11 Calculate the specific activity of step#4. Note that percent recovery=% Yield.