In an experiment the transport of the neurotransmitter serotonin into cultured mammalian cells was measured. Batches of cells were incubated with solutions of different concentrations of radiolabelled [3H]-serotonin for a defined time, cells were then separated from the incubation buffer, and serotonin accumulated into the cells was determined by scintillation counting. The rate of transport is given as dpm/min* (see table below). In order to elucidate the transport mechanism, the experiment was conducted under two different conditions, i.e., in condition A normal transport buffer was used, and in condition B the transport buffer did not contain any Na* ions. [Notes: * dpm/min means disintegrations per minute and is a measure of the intensity of radioactivity of a radiolabelled compound; it is directly related to the rate of transport for this compound] serotonin [µM] 0.025 transport rate A [dpm/min] 300.0 transport rate B [dpm/min] 10 0.08 920.0 30.0 0.25 2500.0 100.0 0.7 5400.0 350.0 2 8800.0 1100.0 6. 9100.0 3000.0
In an experiment the transport of the neurotransmitter serotonin into cultured mammalian cells was measured. Batches of cells were incubated with solutions of different concentrations of radiolabelled [3H]-serotonin for a defined time, cells were then separated from the incubation buffer, and serotonin accumulated into the cells was determined by scintillation counting. The rate of transport is given as dpm/min* (see table below). In order to elucidate the transport mechanism, the experiment was conducted under two different conditions, i.e., in condition A normal transport buffer was used, and in condition B the transport buffer did not contain any Na* ions. [Notes: * dpm/min means disintegrations per minute and is a measure of the intensity of radioactivity of a radiolabelled compound; it is directly related to the rate of transport for this compound] serotonin [µM] 0.025 transport rate A [dpm/min] 300.0 transport rate B [dpm/min] 10 0.08 920.0 30.0 0.25 2500.0 100.0 0.7 5400.0 350.0 2 8800.0 1100.0 6. 9100.0 3000.0
Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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Compare the plots of the transport rates for both conditions. What is most likely
mechanism of transport for serotonin into these cells? Explain your interpretation.
![In an experiment the transport of the neurotransmitter serotonin into cultured mammalian
cells was measured. Batches of cells were incubated with solutions of different
concentrations of radiolabelled [3H]-serotonin for a defined time, cells were then separated
from the incubation buffer, and serotonin accumulated into the cells was determined by
scintillation counting. The rate of transport is given as dpm/min* (see table below). In order
to elucidate the transport mechanism, the experiment was conducted under two different
conditions, i.e., in condition A normal transport buffer was used, and in condition B the
transport buffer did not contain any Na* ions.
[Notes: * dpm/min means disintegrations per minute and is a measure of the intensity of
radioactivity of a radiolabelled compound; it is directly related to the rate of transport for this
compound]
serotonin [µM]
0.025
transport rate A [dpm/min]
300.0
transport rate B [dpm/min]
10
0.08
920.0
30.0
0.25
2500.0
100.0
0.7
5400.0
350.0
2
8800.0
1100.0
6.
9100.0
3000.0](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc052dae8-d4f9-4c1e-9a92-f7b9167ef9ed%2Fe5e47b2a-6107-4fbb-b278-6bd4ba956df4%2Frgwgmz_processed.png&w=3840&q=75)
Transcribed Image Text:In an experiment the transport of the neurotransmitter serotonin into cultured mammalian
cells was measured. Batches of cells were incubated with solutions of different
concentrations of radiolabelled [3H]-serotonin for a defined time, cells were then separated
from the incubation buffer, and serotonin accumulated into the cells was determined by
scintillation counting. The rate of transport is given as dpm/min* (see table below). In order
to elucidate the transport mechanism, the experiment was conducted under two different
conditions, i.e., in condition A normal transport buffer was used, and in condition B the
transport buffer did not contain any Na* ions.
[Notes: * dpm/min means disintegrations per minute and is a measure of the intensity of
radioactivity of a radiolabelled compound; it is directly related to the rate of transport for this
compound]
serotonin [µM]
0.025
transport rate A [dpm/min]
300.0
transport rate B [dpm/min]
10
0.08
920.0
30.0
0.25
2500.0
100.0
0.7
5400.0
350.0
2
8800.0
1100.0
6.
9100.0
3000.0
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