Based on the gel profile shown above, which protocol do you think is the best for the butterfly larvae gDNA isolation? Explain the basis of your choice and why you did not choose the other 3 protocols.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
icon
Concept explainers
Question
100%

helping tags: biochemistry, agarose gel electrophoresis, genomic DNA isolation, gel electrophoresis

Please explain your answer thoroughly. Thanks will upvote.

There are 4 protocols that are being optimized for the isolation of genomic DNA from
the larvae of a newly discovered butterfly species. The results of the isolation
experiments were verified via agarose gel electrophoresis and are shown below:
1kb DNA
Protocol
Protocol
Protocol
Protocol
ladder +
A
B
D
25,000
10,000
8,000
6,000
5,000
4,000
3,000
2,500
2,000
1,500
1,000
750
500
250
Based on the gel profile shown above, which protocol do you think is the best for the
butterfly larvae 9DNA isolation? Explain the basis of your choice and why you did not
choose the other 3 protocols.
Transcribed Image Text:There are 4 protocols that are being optimized for the isolation of genomic DNA from the larvae of a newly discovered butterfly species. The results of the isolation experiments were verified via agarose gel electrophoresis and are shown below: 1kb DNA Protocol Protocol Protocol Protocol ladder + A B D 25,000 10,000 8,000 6,000 5,000 4,000 3,000 2,500 2,000 1,500 1,000 750 500 250 Based on the gel profile shown above, which protocol do you think is the best for the butterfly larvae 9DNA isolation? Explain the basis of your choice and why you did not choose the other 3 protocols.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Molecular techniques
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education