Based on the gel profile shown above, which protocol do you think is the best for the butterfly larvae gDNA isolation? Explain the basis of your choice and why you did not choose the other 3 protocols.
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
helping tags: biochemistry, agarose gel electrophoresis, genomic DNA isolation, gel electrophoresis
Please explain your answer thoroughly. Thanks will upvote.
![There are 4 protocols that are being optimized for the isolation of genomic DNA from
the larvae of a newly discovered butterfly species. The results of the isolation
experiments were verified via agarose gel electrophoresis and are shown below:
1kb DNA
Protocol
Protocol
Protocol
Protocol
ladder +
A
B
D
25,000
10,000
8,000
6,000
5,000
4,000
3,000
2,500
2,000
1,500
1,000
750
500
250
Based on the gel profile shown above, which protocol do you think is the best for the
butterfly larvae 9DNA isolation? Explain the basis of your choice and why you did not
choose the other 3 protocols.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F0a7a77fd-30b4-4c19-b3e9-8b3a92ea531c%2F0b842f72-a60a-4a44-8e20-fb68bec3be10%2Fwe6cpxg_processed.png&w=3840&q=75)
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