Arrange the best steps to take to identify the gene and mutation that causes the lung cancer. Not all of the answers will be placed. Isolate tumor DNA lightly fragment the genome transfect the genome into mouse cell line determine shared transfected region repeat with small fragments and sequence the gene generate a cell line from the tumor sequence the tumor-cell genome isolate transformed colonies introduce transfected cells into a mouse

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Arrange the best steps to take to identify the gene and mutation that causes the lung cancer. Not all of the answers will
be placed.
Isolate tumor DNA
lightly fragment the genome
transfect the genome into mouse cell line
determine shared transfected region
repeat with small fragments and sequence the gene
generate a cell line from the tumor
sequence the tumor-cell genome
isolate transformed colonies
introduce transfected cells into a mouse
Introduce the mutation in cell culture
Transcribed Image Text:Arrange the best steps to take to identify the gene and mutation that causes the lung cancer. Not all of the answers will be placed. Isolate tumor DNA lightly fragment the genome transfect the genome into mouse cell line determine shared transfected region repeat with small fragments and sequence the gene generate a cell line from the tumor sequence the tumor-cell genome isolate transformed colonies introduce transfected cells into a mouse Introduce the mutation in cell culture
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