An important validation of the genetic code occurred when George Streisinger determined the amino acid sequence of bacteriophage T4 lysozyme and of mutants induced by proflavin, a dye with a planar structure that can intercalate (fit) between successive base pairs in DNA and induce frameshift mutations—that is, mutations involving additions or deletions of a single base. Streisinger and colleagues found that a particular single-base insertion mutation could be suppressed, with wild-type function restored, by a mutation that evidently involved a single-base deletion at a nearby site. Shown below are portions of the amino acid sequence of wild-type T4 lysozyme and the putative double mutant. The remaining parts of the sequence were unchanged. Identify mRNA sequences that could encode each of these amino acid sequences and determine whether your codon assignments are consistent with the genetic code.Wild-type …Lys–Ser–Pro–Ser–Leu–Asn–Ala…Double mutant …Lys–Val–His–His–Leu–Met–Ala…
An important validation of the genetic code occurred when George Streisinger determined the amino acid sequence of bacteriophage T4 lysozyme and of mutants induced by proflavin, a dye with a planar structure that can intercalate (fit) between successive base pairs in DNA and induce frameshift mutations—that is, mutations involving additions or deletions of a single base. Streisinger and colleagues found that a particular single-base insertion mutation could be suppressed, with wild-type function restored, by a mutation that evidently involved a single-base deletion at a nearby site. Shown below are portions of the amino acid sequence of wild-type T4 lysozyme and the putative double mutant. The remaining parts of the sequence were unchanged. Identify mRNA sequences that could encode each of these amino acid sequences and determine whether your codon assignments are consistent with the genetic code.
Wild-type …Lys–Ser–Pro–Ser–Leu–Asn–Ala…
Double mutant …Lys–Val–His–His–Leu–Met–Ala…
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