10-¹1 M. A 1 nM (10-⁹ M) solution of lysozyme iso An Fab fragment binds to lysozyme with a dissociation constant of Kåtreated with increasing concentrations of the Fab fragment. At what concentration of added Fab will half of the lysozyme bebound to the Fab?[F] =nM

we can use the equation for the fractional saturation (Y):Y=[F]/Kd+[F]Given:Kd=10−11M[L]=10−9M

Answered: = An Fab fragment binds to lysozyme…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

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Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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= An Fab fragment binds to lysozyme with a dissociation constant of Ka 10-¹1 M. A 1 nM (10-⁹ M) solution of lysozyme is treated with increasing concentrations of the Fab fragment. At what concentration of added Fab will half of the lysozyme be bound to the Fab? [Fab] = nM