An enzyme catalyzes the reaction A --> B. The enzyme is present at aconcentration of 2 nM, and the Vmax is 10 uM/s. The Km for substrate A is10mMCalculate the initial velocity of the reaction, Vo, when the substrateconcentration is a)2 mM and b) 30 mM.

The Michaelis-Menten equation is a mathematical model that describes the relationship between the…

Answered: An enzyme catalyzes the reaction A -->…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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An uncatalyzed reaction has keq=50. in the presence of an appropriate enzyme.the forward rate of the reaction increased by 20-fold.what is the equilibrium constant in the presence of the enzyme?
An enzyme catalyzes the reaction M-N. The enzyme is present at a concentration of 0.0000000022 M, and the Vmax is 0.0000028 Ms1. The Km for substrate is 5.5 µM. Calculate kcat-
In a alkaline phosphatase kinetics experiments a studnet first made a standard curve with different amounts of PNP. In a reaction carried out with 10nM alkaline phosphatase and 100 uM PNPP substrate the student measured an absorbance value of 0.76 at 410nm after 30 seconds of reaction. Assuming the 30 second time point is in the lienar range of the reaction and the substrate has not been depleted at this time point, calculate the Vo at 100uM PNPP (in units of concentration per time).Beer's law formula given is y=0.0323x (y axis is Absorbance) (x Axis is PNP uM)
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An enzyme catalyzes the reaction A --> B. The enzyme is present at a Concentration of 2 nM, and the Vmax is 10 uM/s. The Km for substrate A is 10mM Calculate the initial velocity of the reaction, Vo, when the substrate Concentration is a)2 mM and b) 30 mm.