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Agent Step 1 Step 2 Nucleophile, Nu: Guanosine Leaving group, Y Electrophile ✗, part retained 3'-site phosphate Agent Nucleophile, Nu: Step 1 Step 2 2'01 BPA 3'-OH exon 1 Leaving group, Y 3'OH exon 1 3'0M intron 1 Electrophile X, part retained 5'-site phosphate intron I and extron 1 PO3 with nuclectide? 3'-site phosphate extron (1 and 2) POS with nucleotice?

Agent Step 1 Step 2 Nucleophile, Nu: Guanosine Leaving group, Y Electrophile ✗, part retained 3'-site phosphate Agent Nucleophile, Nu: Step 1 Step 2 2'01 BPA 3'-OH exon 1 Leaving group, Y 3'OH exon 1 3'0M intron 1 Electrophile X, part retained 5'-site phosphate intron I and extron 1 PO3 with nuclectide? 3'-site phosphate extron (1 and 2) POS with nucleotice?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question 2:

Part a: Complete the table describing different components of intron removal from mRNA. Nu:, X and Y refer to B-type chemistry shown on the previous page. (YELLOW table shown)

Part b: Complete the table describing different components of group I self-splicing intron removal from 26S rRNA in Tetrahymena. (BLUE table shown)

Part c: Draw the intron with an all atom structure for Branchpoint A after intron removal from mRNA

Part d: Draw the Group I self-splicing intron with an all atom structure for the Guanosine cofactor after intron removal from 26S rRNA in Tetrahymena.

 

Agent Step 1 Step 2 Nucleophile, Nu: Guanosine Leaving group, Y Electrophile ✗, part retained 3'-site phosphate
Transcribed Image Text:Agent Step 1 Step 2 Nucleophile, Nu: Guanosine Leaving group, Y Electrophile ✗, part retained 3'-site phosphate
Agent Nucleophile, Nu: Step 1 Step 2 2'01 BPA 3'-OH exon 1 Leaving group, Y 3'OH exon 1 3'0M intron 1 Electrophile X, part retained 5'-site phosphate intron I and extron 1 PO3 with nuclectide? 3'-site phosphate extron (1 and 2) POS with nucleotice?
Transcribed Image Text:Agent Nucleophile, Nu: Step 1 Step 2 2'01 BPA 3'-OH exon 1 Leaving group, Y 3'OH exon 1 3'0M intron 1 Electrophile X, part retained 5'-site phosphate intron I and extron 1 PO3 with nuclectide? 3'-site phosphate extron (1 and 2) POS with nucleotice?
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