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Absorbance 2.0 1.8- 16 1.4 1.2 1.0 ast 0.2 Aap The Ser Glu Cly Val Me Tye Waln Ha LA 7 8 9 10 11 12 13 14 15 16 Effunt, mL (Flow rate-12mL/hr) (a1) Why Asp preceeds Ser/Thr? (a2) Why Ser/Thr preceed Val/Met/Leu/llu (isoleucine)? (a3) Why Val/Met/Leu/llu (isoleucine) preceed Tyr/Phe? -CH₂-CH-CH₂-CH- $0₂ SO₂ Chemical structure of resin polymer

Absorbance 2.0 1.8- 16 1.4 1.2 1.0 ast 0.2 Aap The Ser Glu Cly Val Me Tye Waln Ha LA 7 8 9 10 11 12 13 14 15 16 Effunt, mL (Flow rate-12mL/hr) (a1) Why Asp preceeds Ser/Thr? (a2) Why Ser/Thr preceed Val/Met/Leu/llu (isoleucine)? (a3) Why Val/Met/Leu/llu (isoleucine) preceed Tyr/Phe? -CH₂-CH-CH₂-CH- $0₂ SO₂ Chemical structure of resin polymer

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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In ion-exchange chromatography, amino acids are separated on the basis of electro-
static interactions and hydrophobic interactions with the resin. The chemical structure of the resin pol-
ymer with a negatively charged sulfonic acid group is shown to the right. An elution profile for the amino
acids using this resin is given below. Based on the elution profile above, explain the following:

Absorbance 2.0 1.8- 1.6 1.0 0.3 0.5 0.0 0.2 Ap The Ser Glu Gly |Val Mes Les Tye املات Ha LA NH₂ 7 8 9 10 11 12 13 14 15 16 Effunt, mL (Flow rate-12mL/hr) (a1) Why Asp preceeds Ser/Thr? (a2) Why Ser/Thr preceed Val/Met/Leu/llu (isoleucine)? (a3) Why Val/Met/Leu/llu (isoleucine) preceed Tyr/Phe? -CH₂-CH-CH₂-CH- SO₂ Chemical structure of resin polymer SO
Transcribed Image Text:Absorbance 2.0 1.8- 1.6 1.0 0.3 0.5 0.0 0.2 Ap The Ser Glu Gly |Val Mes Les Tye املات Ha LA NH₂ 7 8 9 10 11 12 13 14 15 16 Effunt, mL (Flow rate-12mL/hr) (a1) Why Asp preceeds Ser/Thr? (a2) Why Ser/Thr preceed Val/Met/Leu/llu (isoleucine)? (a3) Why Val/Met/Leu/llu (isoleucine) preceed Tyr/Phe? -CH₂-CH-CH₂-CH- SO₂ Chemical structure of resin polymer SO
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