Abosrbance (A 280) You are interested in isolating an enzyme Ribonuclease. Inside cells ribonuclease self-associates and can exist as dimers or trimers. The active form of the enzyme is monomeric with molecular weight of ~ 14Kda. In order to isolate ribonuclease, you use size exclusion chromatography column and obtain three peaks. Indicate which peak (starting from left to right) corresponds to Monomer (M), Dimer (D) and Trimer (T) forms of ribonuclease. PEAK I: PEAK II: PEAK III: Time м Fractions

Q: What is a Ramachandran Plot?

A: Ramachandran plot is developed by G N Ramachandran, C Ramakrishnan and V Sasisekharan in 1963. This…

Q: What is the importance of having a weak chemical bond? - Cite one practical use.

A: A compound is formed by combining of many elements. Each element joins with another element with the…

Q: 13. Shape and dimension. (a) Tropomyosın, a 70-kDa muscle protein, is a two-stranded a-helical…

A: Secondary structure of protein: Formed due to twisting of the polypeptide chain.The folding is due…

Q: Can you help find the c terminal at ph 9.5?

A: The given hexapeptide is composed of serine, histidine, glutamate, cysteine, arginine, and…

Q: If a protein with the sequence AGFEWPRQVDMARINE is treated with chymotrypsin, what will the products…

A: Proteins are made up of amino acids. Two amino acids are joined by a peptide bond in a protein.…

Q: calculate P1 for amino acid Tryptophan. Also please check if I draw the mechanism correctly and…

A: The proteins are constituted of 20 naturally occurring amino acids. The amino acid Tryptophan is an…

Q: A protein gives, under conditions of buffer composition, pH, and temperature that are close to…

A: SDS (Sodium dodecyl sulfate) breaks non-covalent interactions like hydrogen bonds and ionic bonds…

Q: Draw one of the possible dipeptides that could be formed between serine and lysine. Add charges to…

A: Amino acids are organic compounds charaterized by the presence of two functional groups in their…

Q: The peptide below H3N-CH- I NH–CH -NH-CH -NH-CH CH3 CH2 CH2 CH2 CH2 H₂C-CH HỌCH, NH–CH CH2 OH -OH…

A: Peptides are composed of amino acids. Ionizable groups in a peptide are the N-terminal alpha-amino…

Q: Base pairs Data – all had Temp = 250C PH = 7 Binding Constant A & X [A] = 0.00373221M [X] =…

A: The DNA double helix is made of two linked strands that are wound in a helical shape. The two…

Q: 3. To the right is a Lineweaver-Burk plot for enzyme X along with two additional lines from testing…

A: Michaelis Menten constant for enzymatic activities, is a concentration of a substrate which is…

Q: Is RNA-binding enzyme RNase A more likely to have a pI of 9.2 or 5.0?Briefly explain your reasoning.

A: Ribonuclease A (RNase A) is a digestive enzyme secreted by the pancreas that specifically digests or…

Q: starting and ending purity, t

A: Purification of a Recombinant Protein:This process involves the following steps-Cel harvesting- In…

Q: Test Case 1: Below is a file that contains two different alignments (separated by a '#') for the…

A:

Q: Carbohydrates: Draw the structures of the two products obtained when lactose is subjected to…

A: Lactose is a disaccharide composed of a  and a . The C1 of  forms a glycosidic bond with C4 of…

Q: 4.08 H H ↓ 1.02 ↓ | 2.54 11.02↑ E E Note: 1.67 = EXON = INTRON E 10.8 kbp 3.94 3.66 E = EcoRI site H…

A: Endonuclease HindIII is a type II restriction enzyme that recognizes and cleaves the palindromic…

Q: stion.} Br CI CI in the blank by typing the correct complete IUPAC name in the bo e correct complete…

A:

Q: 13.13) Write the names (using the three letter abbreviation method) of all of the tripeptides that…

A: Tripeptide is the macromolecule formed from three amino acids. Amino acids are joined by peptide…

Q: affinity

A:

Q: ✓ Part B What further experiment should be carried out in order to determine the primary structure…

A: Introduction Protein is the most abundant macromolecule in our body.  Amino acids are the building…

Q: Draw the structure of the dipeptide that is formed at physiological pH when alanine is the…

A: A dipeptide is an atom made up of two amino acids connected together. Alanine being the N-terminal,…

Q: Consider a small protein containing 98 amino acid residues. The protein backbone will have 194 bonds…

A: Assuming that each of the 194 rotatable links in the protein backbone can have three alternative…

Q: H,C NH H. of HO-P-O- HO-P-O- OH ÓH ОН ОН OH C D N- NH O

A: Nucleotides are the elemental building blocks of nucleic acids. ribonucleic acid and…

Q: A protein has been sequenced after cleavage of disulfide bonds. The protein is known to contain 3…

A: There are four classes of biological macromolecules: Nucleic acids, Proteins, Lipids and…

Q: 2+ ions 6.46 The following data show the binding of Mg2+ with a protein containing n equivalent…

A: The Scatchard equation is a mathematical equation that is used to determine the binding of ligands…

Q: Predict the number of bands and apparent mol. wt. of the following proteins on SDS gels. 1. A…

A: On basis of the gel used, electrophoresis is classified into 2 types, Agarose gel…

Q: Explain your reasoning for your proposed synthesis of your choice. Your explanation should touch…

A:

Q: Plus pole pH 11.0 Middle of Paper Minus pole Aspartate Lysine Leucine Histidine Example amino acic

A: Proteins are large, complex molecules that are essential to the structure and function of cells,…

Q: 3. A. Briefly discuss the four levels of structure in proteins. Knowing that the 3-dimensional shape…

A: Protein is a polypeptide made up of amino acids. The sequence of amino acids determines the…

Q: Amino acid analysis of a HEPTAPEPTIDE reveals the following information below: (NOTE: when the…

A: The proteins are composed of sequence of amino acids connected via peptide bonds. The sequence of…