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A sample (1.90 x 10² mg) of an oligomeric protein of M, 113,000 was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) under slightly alkaline conditions until the chemical reaction was complete. The peptide bonds of the protein were then completely hydrolyzed by heating it with concentrated HCl. The hydrolysate was found to contain 2.85 mg of DNP-Val. 2,4-Dinitrophenyl derivatives of the a-amino groups of other amino acids could not be found. Calculate the number of polypeptide chains in this protein. Give the answer as a whole number. number of polypeptide chains: 3.9 DNP-Val NO₂ 2 NH Sx C CH H₂C O,N COOH H CH₂

A sample (1.90 x 10² mg) of an oligomeric protein of M, 113,000 was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) under slightly alkaline conditions until the chemical reaction was complete. The peptide bonds of the protein were then completely hydrolyzed by heating it with concentrated HCl. The hydrolysate was found to contain 2.85 mg of DNP-Val. 2,4-Dinitrophenyl derivatives of the a-amino groups of other amino acids could not be found. Calculate the number of polypeptide chains in this protein. Give the answer as a whole number. number of polypeptide chains: 3.9 DNP-Val NO₂ 2 NH Sx C CH H₂C O,N COOH H CH₂

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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A sample (1.90 x 102 mg) of an oligomeric protein of M, 113,000 was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) under slightly alkaline conditions until the chemical reaction was complete. The peptide bonds of the protein were then completely hydrolyzed by heating it with concentrated HCl. The hydrolysate was found to contain 2.85 mg of DNP-Val. 2,4-Dinitrophenyl derivatives of the a-amino groups of other amino acids could not be found. Calculate the number of polypeptide chains in this protein. Give the answer as a whole number. number of polypeptide chains: 3.9 Incorrect O₂N DNP-Val NO₂ NH H₂C CH COOH H CH₂
Transcribed Image Text:A sample (1.90 x 102 mg) of an oligomeric protein of M, 113,000 was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) under slightly alkaline conditions until the chemical reaction was complete. The peptide bonds of the protein were then completely hydrolyzed by heating it with concentrated HCl. The hydrolysate was found to contain 2.85 mg of DNP-Val. 2,4-Dinitrophenyl derivatives of the a-amino groups of other amino acids could not be found. Calculate the number of polypeptide chains in this protein. Give the answer as a whole number. number of polypeptide chains: 3.9 Incorrect O₂N DNP-Val NO₂ NH H₂C CH COOH H CH₂
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