Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
A sample (1.90 x 10² mg) of an oligomeric protein of M, 113,000 was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) under slightly alkaline conditions until the chemical reaction was complete. The peptide bonds of the protein were then completely hydrolyzed by heating it with concentrated HCl. The hydrolysate was found to contain 2.85 mg of DNP-Val. 2,4-Dinitrophenyl derivatives of the a-amino groups of other amino acids could not be found. Calculate the number of polypeptide chains in this protein. Give the answer as a whole number. number of polypeptide chains: 3.9 DNP-Val NO₂ 2 NH Sx C CH H₂C O,N COOH H CH₂

A sample (1.90 x 10² mg) of an oligomeric protein of M, 113,000 was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) under slightly alkaline conditions until the chemical reaction was complete. The peptide bonds of the protein were then completely hydrolyzed by heating it with concentrated HCl. The hydrolysate was found to contain 2.85 mg of DNP-Val. 2,4-Dinitrophenyl derivatives of the a-amino groups of other amino acids could not be found. Calculate the number of polypeptide chains in this protein. Give the answer as a whole number. number of polypeptide chains: 3.9 DNP-Val NO₂ 2 NH Sx C CH H₂C O,N COOH H CH₂

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question

Do not give handwriting solution.

A sample (1.90 x 102 mg) of an oligomeric protein of M, 113,000 was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) under slightly alkaline conditions until the chemical reaction was complete. The peptide bonds of the protein were then completely hydrolyzed by heating it with concentrated HCl. The hydrolysate was found to contain 2.85 mg of DNP-Val. 2,4-Dinitrophenyl derivatives of the a-amino groups of other amino acids could not be found. Calculate the number of polypeptide chains in this protein. Give the answer as a whole number. number of polypeptide chains: 3.9 Incorrect O₂N DNP-Val NO₂ NH H₂C CH COOH H CH₂
Transcribed Image Text:A sample (1.90 x 102 mg) of an oligomeric protein of M, 113,000 was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) under slightly alkaline conditions until the chemical reaction was complete. The peptide bonds of the protein were then completely hydrolyzed by heating it with concentrated HCl. The hydrolysate was found to contain 2.85 mg of DNP-Val. 2,4-Dinitrophenyl derivatives of the a-amino groups of other amino acids could not be found. Calculate the number of polypeptide chains in this protein. Give the answer as a whole number. number of polypeptide chains: 3.9 Incorrect O₂N DNP-Val NO₂ NH H₂C CH COOH H CH₂
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS