A. Dr. Randy Schekman introduced you to a yeast model system for studying membrane fusion and vesicle trafficking. Imagine that you have isolated a new temperature-sensitive sec mutant which you suspect has a specific defect in secretory vesicle docking and fusion with the plasma membrane (exocytosis). Describe an experiment you could do to test your hypothesis. B. Now, explain how you would continue your experiment to identify which secretory pathway proteins are present on these secretory vesicles. C. Finally, among the proteins present in these synaptic vesicles according to mass spec you find a protein which contains a G-domain fold suggesting that it is a G-protein. Briefly outline how, in principle, you would determine which of the other proteins is a GAP for this G protein.
A. Dr. Randy Schekman introduced you to a yeast model system for studying membrane fusion and vesicle trafficking. Imagine that you have isolated a new temperature-sensitive sec mutant which you suspect has a specific defect in secretory vesicle docking and fusion with the plasma membrane (exocytosis). Describe an experiment you could do to test your hypothesis.
B. Now, explain how you would continue your experiment to identify which secretory pathway proteins are present on these secretory vesicles.
C. Finally, among the proteins present in these synaptic vesicles according to mass spec you find a protein which contains a G-domain fold suggesting that it is a G-protein. Briefly outline how, in principle, you would determine which of the other proteins is a GAP for this G protein.
Step by step
Solved in 3 steps