A unique plant was discovered from the hot springs of Maquinit, Coron, Palawan. To study the activity of the plant, approximately 5.00 g of the fresh plant was finely cut and ground and then mixed with an extraction buffer to make up a total of 1.00 mL crude plant extract. Approximately 19.00 mL of water was added to the plant extract. To determine the protein concentration of the extract, a bovine serum albumin (BSA) stock solution with a concentration of 300 μg mL-1 was used and mixtures with the following compositions and absorbance readings were prepared: A 100 μL aliquot of the diluted plant extract was added with 400 μL of water and was subjected to Bradford assay. Determine the protein concentration, in mg mL-1, of the original 1.00 mL crude extract if the absorbance of the diluted aliquot extract acquired from the analysis is 0.234.
A unique plant was discovered from the hot springs of Maquinit, Coron, Palawan. To study the activity of the plant, approximately 5.00 g of the fresh plant was finely cut and ground and then mixed with an extraction buffer to make up a total of 1.00 mL crude plant extract. Approximately 19.00 mL of water was added to the plant extract. To determine the protein concentration of the extract, a bovine serum albumin (BSA) stock solution with a concentration of 300 μg mL-1 was used and mixtures with the following compositions and absorbance readings were prepared: A 100 μL aliquot of the diluted plant extract was added with 400 μL of water and was subjected to Bradford assay. Determine the protein concentration, in mg mL-1, of the original 1.00 mL crude extract if the absorbance of the diluted aliquot extract acquired from the analysis is 0.234.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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A unique plant was discovered from the hot springs of Maquinit, Coron, Palawan. To study the activity of the plant, approximately 5.00 g of the fresh plant was finely cut and ground and then mixed with an extraction buffer to make up a total of 1.00 mL crude plant extract. Approximately 19.00 mL of water was added to the plant extract. To determine the protein concentration of the extract, a bovine serum albumin (BSA) stock solution with a concentration of 300 μg mL-1 was used and mixtures with the following compositions and absorbance readings were prepared:
A 100 μL aliquot of the diluted plant extract was added with 400 μL of water and was subjected to Bradford assay. Determine the protein concentration, in mg mL-1, of the original 1.00 mL crude extract if the absorbance of the diluted aliquot extract acquired from the analysis is 0.234.
Transcribed Image Text:Tube #
Volume of BSA, mL
Volume of water, mL
Absorbance
1
0.00
3.00
0.000
0.50
2.50
0.105
3.
1.00
2.00
0.203
1.50
1.50
0.298
5
2.00
1.00
0.385
6
3.00
0.00
0.475
4-
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