Suppose that you have an enzyme concentration at the molarity calculated in question 2 and the product that you monitor has a value of x = 6200 M1 cm1. You ran eight reactions and found the following: Reaction # [substrate] (μM) Slope (Abs/min) 0 0.000 2345678 10 0.163 20 0.288 60 0.586 100 0.740 200 0.920 500 1.078 1000 1.143 M-4.45x10-M Calculate the values of Kcat and KM for the reaction (remember to include units). Show your plot and your calculations.
Suppose that you have an enzyme concentration at the molarity calculated in question 2 and the product that you monitor has a value of x = 6200 M1 cm1. You ran eight reactions and found the following: Reaction # [substrate] (μM) Slope (Abs/min) 0 0.000 2345678 10 0.163 20 0.288 60 0.586 100 0.740 200 0.920 500 1.078 1000 1.143 M-4.45x10-M Calculate the values of Kcat and KM for the reaction (remember to include units). Show your plot and your calculations.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
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![Suppose that you have an enzyme concentration at the molarity calculated in question 2 and
the product that you monitor has a value of x = 6200 M1 cm1. You ran eight reactions and found
the following:
Reaction #
[substrate] (μM)
Slope (Abs/min)
0
0.000
2345678
10
0.163
20
0.288
60
0.586
100
0.740
200
0.920
500
1.078
1000
1.143
M-4.45x10-M
Calculate the values of Kcat and KM for the reaction (remember to include units). Show your plot
and your calculations.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F91d53b99-75a0-4350-93b8-88d6398b6959%2Fbcffe5cf-7286-4945-bca6-b90ff6a5de76%2F2rrb27q_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Suppose that you have an enzyme concentration at the molarity calculated in question 2 and
the product that you monitor has a value of x = 6200 M1 cm1. You ran eight reactions and found
the following:
Reaction #
[substrate] (μM)
Slope (Abs/min)
0
0.000
2345678
10
0.163
20
0.288
60
0.586
100
0.740
200
0.920
500
1.078
1000
1.143
M-4.45x10-M
Calculate the values of Kcat and KM for the reaction (remember to include units). Show your plot
and your calculations.
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