Suppose that you have an enzyme concentration at the molarity calculated in question 2 and the product that you monitor has a value of x = 6200 M1 cm1. You ran eight reactions and found the following: Reaction # [substrate] (μM) Slope (Abs/min) 0 0.000 2345678 10 0.163 20 0.288 60 0.586 100 0.740 200 0.920 500 1.078 1000 1.143 M-4.45x10-M Calculate the values of Kcat and KM for the reaction (remember to include units). Show your plot and your calculations.

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Suppose that you have an enzyme concentration at the molarity calculated in question 2 and
the product that you monitor has a value of x = 6200 M1 cm1. You ran eight reactions and found
the following:
Reaction #
[substrate] (μM)
Slope (Abs/min)
0
0.000
2345678
10
0.163
20
0.288
60
0.586
100
0.740
200
0.920
500
1.078
1000
1.143
M-4.45x10-M
Calculate the values of Kcat and KM for the reaction (remember to include units). Show your plot
and your calculations.
Transcribed Image Text:Suppose that you have an enzyme concentration at the molarity calculated in question 2 and the product that you monitor has a value of x = 6200 M1 cm1. You ran eight reactions and found the following: Reaction # [substrate] (μM) Slope (Abs/min) 0 0.000 2345678 10 0.163 20 0.288 60 0.586 100 0.740 200 0.920 500 1.078 1000 1.143 M-4.45x10-M Calculate the values of Kcat and KM for the reaction (remember to include units). Show your plot and your calculations.
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