A student wants to prepare a standard solution of BSA. They create a stock solution of BSA by add O.174 g of solid BSA powder into a 10 ml volumetric flask, then fills the flask to the line with 0.1M PBS buffer. The student uses a volumetric pipet to transfer 4 ml of stock solution into a new 10 ml volumetric flask, then fills the flask to the calibration line with 0.1M PBS buffer, and calls this Dilution 1. The student uses a micropipette to transfer 23 ul of Dilution 1 and 55 ul of 0.1M PBS butfer into a cuvette. Assuming the volumes are additive, what is the concentration of BSA in the cuvette in mg/mL? Report your answer with two places after the decimal.

A student wants to prepare a standard solution of BSA. They create a stock solution of BSA by add O.174 g of solid BSA powder into a 10 ml volumetric flask, then fills the flask to the line with 0.1M PBS buffer. The student uses a volumetric pipet to transfer 4 ml of stock solution into a new 10 ml volumetric flask, then fills the flask to the calibration line with 0.1M PBS buffer, and calls this Dilution 1. The student uses a micropipette to transfer 23 ul of Dilution 1 and 55 ul of 0.1M PBS butfer into a cuvette. Assuming the volumes are additive, what is the concentration of BSA in the cuvette in mg/mL? Report your answer with two places after the decimal.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

A student wants to prepare a standard solution of BSA. They create a stock solution of BSA by add 0.174 g of solid BSA powder into a 10 ml volumetric flask, then
fills the flask to the line with 0.1M PBS buffer. The student uses a volumetric pipet to transfer 4 ml of stock solution into a new 10 ml volumetric flask, then fills the
flask to the calibration line with 0.1 M PBS buffer, and calls this Dilution 1. The student uses a micropipette to transfer 23 ul of Dilution 1 and 55 ul of Q.1M PBS
buffer into a cuvette. Assuming the volumes are additive, what is the concentration of BSA in the cuvette in mg/mL? Report your answer with two places after the
decimal

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