A similar experiment is as follows: 1. Obtain 11 (50-ml capacity) beakers and mark from 1-11. In each beaker add 20 ml of one concentration of the following sucrose solutions: Distilled water, 0.1., 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0 M 3. 2. Using a cork borer, cut 55 cylinders from a large potato or any storage tissue and trim each cylinder to 0.5 cm in length. Place the cylinders in a covered beaker as you cut them to prevent them from drying up. Divide the cylinders in sets of 5. Weigh each set (to 0.01 g). Put one set of cylinders in each of the beakers. Record the fresh weights of each set in Table 6. 4. 5. After 90 minutes, remove the cylinders, gently blot dry with paper towels and reweigh. 7. 6. Subtract the initial weights from the final weights. Divide the difference by the initial weight and multiply by 100 to get the % weight change. Complete Table 6. 8. Plot the% change in weight (y, ordinate) vs. sucrose concentration (x, abscissa) on a graph. Draw the best fit straight line through the points. Table 6a. Sucrose Concentration 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 (Molarity) 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.0 Initial Weight (g) 3.61 3.38 3.10 3.02 2.93 2.70 2.50 1.90 1.80 1.631.38 Final Weight (g) A Weight (g) % Weight Change

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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• Estimation of the Water Potential (Ψw) of Storage Tissue by Volume Change Method Observe these experiments in these links: https://www.youtube.com/watch?v=jTDATlaBV-o https://www.youtube.com/watch?v=k1O9jBHgsxs Estimates of water potential of various tissues have been obtained after equilibrating the tissues in solutions of different osmotic potentials. The principle depends upon finding the concentration of sucrose that would cause no change in weight of the tissue before and after an incubation period in the solution. This concentration may be assumed to have a water potential equal to that of the tissue.

A similar experiment is as follows:
1.
Obtain 11 (50-ml capacity) beakers and mark
from 1-11.
In each beaker add 20 ml of one concentration of
the following sucrose solutions: Distilled water, 0.1.,
0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0 M
3.
2.
Using a cork borer, cut 55 cylinders from a large
potato or any storage tissue and trim each cylinder to
0.5 cm in length.
Place the cylinders in a covered beaker as you
cut them to prevent them from drying up.
Divide the cylinders in sets of 5. Weigh each set
(to 0.01 g). Put one set of cylinders in each of the
beakers.
4.
5.
Record the fresh weights of each set in
Table 6.
After 90 minutes, remove the cylinders, gently
blot dry with paper towels and reweigh.
7.
6.
Subtract the initial weights from the final weights.
Divide the difference by the initial weight and multiply
by 100 to get the % weight change. Complete Table 6.
Plot the% change in weight (y, ordinate) vs.
sucrose concentration (x, abscissa) on a graph. Draw
the best fit straight line through the points.
8.
Table 6а.
Sucrose
Concentration
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0
(Molarity)
|3.00|3.00|3.00|이3.00|3.00|3.00|3.00|3.00|3.00|3.00| 3.0
Initial Weight (g)
3.61 3.38 3.103.02 2.93 2.70 2.50 1.90 1.80 1.63 1.38
Final Weight (g)
A Weight (g)
% Weight Change
Table 6b.
Sucrose
Concentration
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0
(Molarity)
5.00 5.00 5.005.00 5.00 5.00 5.00 5.00 5.00 5.00 5.0
Initial Weight (g)
|6.41 6.38 5.80 5.05 4.50 4.00 3.50 2.90 2.20 1.75 1.38
Final Weight (g)
A Weight (g)
% Weight
Change

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