A researcher used polymerase chain reaction (PCR) to test four individual nematodes for the presence of a mutation in Gene Y. When amplified, the fragments of DNA containing the mutated gene alleles were 250 base pairs long and the normal (wild type) alleles were 350 base pairs long. To identify the genotypes of the individual nematodes, the researcher used gel electrophoresis to visualize the PCR results. 1000 bp s00 bp 300 bp 200 bp 100 bp Remember that these nematodes are diploid organisms and as such, inherited two copies of Gene Y. Which of the following statements is a correct analysis of these results ) a) Nematode As homozygous for this gene and has two copies of the mutated allele. O b) Nematode C is heterozygous for this gene and has one copy of the mutated allele and one copy of the normal (wild type) allele. OC) Nematode Dis heterozygous for this gene and has one copy of the mutated allele and one copy of the normal (wild type) allele. O d) Nematode B is homozygous for this gene and has two copies of the normal (wild type) allele. Nematode A Nematode B Nematode C Nematode D DNA ladder
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
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