A researcher studying a line of cancer cells noticed that cellular content of RhoBTB3 and Cyclin E1 increase and decrease through the cell cycle.a. He hypothesizes that RhoBTB3 signaling regulates the production of Cyclin E1 and the entry of the cell into mitosis. Based on the data below, explain why his results do not support this hypothesis. Identify what conclusions are supported by this data. b. Suppose that the researcher can engineer a cell line in which RhoBTB3 expression can be induced by the addition of a drug. Design an experiment using this cell line to test whether RhoBTB3 regulates the production of Cyclin E1. Describe what results he can expect if his hypothesis is correct. **Graph Description:**The graph displays protein levels of Cyclin E1 and RhoBTB3 over time, measured in hours (h). The y-axis represents the protein level in arbitrary units (AU), ranging from 0 to 1, while the x-axis shows time in hours from 0 to 18.- **Cyclin E1** (solid line): Protein levels start at 0, increase sharply from 8 hours, peak around 12 hours, and then gradually decline by 18 hours.- **RhoBTB3** (dashed line): Protein levels remain relatively steady throughout the 0 to 18-hour period.**Western Blot Analysis:**Below the graph, a Western Blot analysis shows the expression of RhoBTB3 and Cyclin E1 across various cell cycle phases: M, G1, S, and G2.- **RhoBTB3**: Consistent protein bands across all phases indicate stable expression levels.- **Cyclin E1**: Noticeable protein bands during S and G2 phases, reflecting the increase in protein levels as seen in the graph.This data helps in understanding the regulation and function of these proteins during the cell cycle.

The cell cycle includes events that terminates with the division of cells. The division process is…

Answered: A researcher studying a line of cancer…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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A researcher studying a line of cancer cells noticed that cellular content of RhoBTB3 and Cyclin E1 increase and decrease through the cell cycle. a. He hypothesizes that RhoBTB3 signaling regulates the production of Cyclin E1 and the entry of the cell into mitosis. Based on the data below, explain why his results do not support this hypothesis. Identify what conclusions are supported by this data. b. Suppose that the researcher can engineer a cell line in which RhoBTB3 expression can be induced by the addition of a drug. Design an experiment using this cell line to test whether RhoBTB3 regulates the production of Cyclin E1. Describe what results he can expect if his hypothesis is correct.

**Graph Description:**

The graph displays protein levels of Cyclin E1 and RhoBTB3 over time, measured in hours (h). The y-axis represents the protein level in arbitrary units (AU), ranging from 0 to 1, while the x-axis shows time in hours from 0 to 18.

- **Cyclin E1** (solid line): Protein levels start at 0, increase sharply from 8 hours, peak around 12 hours, and then gradually decline by 18 hours.
- **RhoBTB3** (dashed line): Protein levels remain relatively steady throughout the 0 to 18-hour period.

**Western Blot Analysis:**

Below the graph, a Western Blot analysis shows the expression of RhoBTB3 and Cyclin E1 across various cell cycle phases: M, G1, S, and G2.

- **RhoBTB3**: Consistent protein bands across all phases indicate stable expression levels.
- **Cyclin E1**: Noticeable protein bands during S and G2 phases, reflecting the increase in protein levels as seen in the graph.

This data helps in understanding the regulation and function of these proteins during the cell cycle.

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