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According to the question，how do we know if we see more than 2 copies ，it indicate the cell is undergoing S phrase？How about when cell is undergoing mitosis？How many fluorescent spots would we see？？I am confused.

According to the question，how do we know if we see more than 2 copies ，it indicate the cell is undergoing S phrase？How about when cell is undergoing mitosis？How many fluorescent spots would we see？？I am confused.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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According to the question，how do we know if we see more than 2 copies ，it indicate the cell is undergoing S phrase？How about when cell is undergoing mitosis？How many fluorescent spots would we see？？I am confused.
Test FISH. 3. You prepare a DNA probe for fluorescence in situ hybridization with a specific gene in human somatic cells. You expect this probe to bind along the chromosomes where the gene is. When you image the nuclei of these cells, you can see 8 distinct fluorescent spots. Assuming your probe did not bind any off-target sequences, can you explain these results? Nonsecific In a normal somatic cell you should have two copies of any given gene, so two spots by FISH. If you see more than 2 copies, perhaps the cell underwent the S phase of the cell cycle twice without dividing through mitosis. (Some cells do this and become polyploid!)
Transcribed Image Text:Test FISH. 3. You prepare a DNA probe for fluorescence in situ hybridization with a specific gene in human somatic cells. You expect this probe to bind along the chromosomes where the gene is. When you image the nuclei of these cells, you can see 8 distinct fluorescent spots. Assuming your probe did not bind any off-target sequences, can you explain these results? Nonsecific In a normal somatic cell you should have two copies of any given gene, so two spots by FISH. If you see more than 2 copies, perhaps the cell underwent the S phase of the cell cycle twice without dividing through mitosis. (Some cells do this and become polyploid!)
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