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A researcher resolves a mixture of peptides using isoelectric focusing. Order the peptides based on their relative positions in the immobilized pH gradient strip at the end of the experiment. Low pH Glu-Gly-Glu-Asp Asp-Ala-Leu-Asp Arg-Ala-Lys-Lys Arg-Gly-Glu-Lys Arg-Leu-Ala-Arg High pH

A researcher resolves a mixture of peptides using isoelectric focusing. Order the peptides based on their relative positions in the immobilized pH gradient strip at the end of the experiment. Low pH Glu-Gly-Glu-Asp Asp-Ala-Leu-Asp Arg-Ala-Lys-Lys Arg-Gly-Glu-Lys Arg-Leu-Ala-Arg High pH

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question 11. // Hint: Isoelectric focusing separates proteins based on their pI values, and can separate proteins that only differ by a net charge of ±1.±1. Recall that an amino acid residue with a negatively charged R group has a relatively low isoelectric point (pI) where it has zero net charge. Likewise, an amino acid residue with a positively charged R group has a relatively high isoelectric point (pI) where it has zero net charge. 

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A researcher resolves a mixture of peptides using isoelectric focusing. Order the peptides based on their relative positions in the immobilized pH gradient strip at the end of the experiment. Low pH Glu-Gly-Glu-Asp Asp-Ala-Leu-Asp Arg-Ala-Lys-Lys Arg-Gly-Glu-Lys Arg-Leu-Ala-Arg High pH
Transcribed Image Text:A researcher resolves a mixture of peptides using isoelectric focusing. Order the peptides based on their relative positions in the immobilized pH gradient strip at the end of the experiment. Low pH Glu-Gly-Glu-Asp Asp-Ala-Leu-Asp Arg-Ala-Lys-Lys Arg-Gly-Glu-Lys Arg-Leu-Ala-Arg High pH
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