A polypeptide is subjected to the following digestion procedures and the fragments are sequenced. What is the sequence of the original peptide? Trypsin treatment Asn-Thr-Trp-Met-Ile-Lys Gly-Tyr-Met-Gln-Phe Val-Leu-Gly-Met-Ser-Arg Cyanogen bromide treatment Gln-Phe Val-Leu-Gly-Met Ile-Lys-Gly-Tyr-Met Ser-Arg-Asn-Thr-Trp-Met
A polypeptide is subjected to the following digestion procedures and the fragments are sequenced. What is the sequence of the original peptide? Trypsin treatment Asn-Thr-Trp-Met-Ile-Lys Gly-Tyr-Met-Gln-Phe Val-Leu-Gly-Met-Ser-Arg Cyanogen bromide treatment Gln-Phe Val-Leu-Gly-Met Ile-Lys-Gly-Tyr-Met Ser-Arg-Asn-Thr-Trp-Met
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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A polypeptide is subjected to the following digestion procedures and the fragments are sequenced. What is the sequence of the original peptide?
Trypsin treatment
Asn-Thr-Trp-Met-Ile-Lys
Gly-Tyr-Met-Gln-Phe
Val-Leu-Gly-Met-Ser-Arg
Cyanogen bromide treatment
Gln-Phe
Val-Leu-Gly-Met
Ile-Lys-Gly-Tyr-Met
Ser-Arg-Asn-Thr-Trp-Met
What is the sequence of the peptide? (use the 3 letter code joined by "dash" ex. Ser-Arg-Asn-Thr-Trp-Met)
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