A mixture of three components is separated using paper chromatography, and each component is analyzed using absorption. With standards containing 100 ppm of each component run simultaneously, the following data were obtained: cmpd A, 0.72 absorbance; B, 0.55; C, 0.80. For the unknown mixture, the data obtined when run under identic conditions were as follows: cmpd A, .0.15 absorbance, B, 0.84; C, 0.75. a) Calculate the relative percentage composition of the unknown mixture. b) The distances traveled by the three components were 8.5, 6.3, and 5.5 cm, respectively. Sketch the appearance of the paper chromatogram. Label each spot. e Colouloto the Df voluOc if the colvent front ic 1 O om

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A mixture of three components is separated using paper chromatography, and each component is analyzed using
absorption. With standards containing 100 ppm of each component run simultaneously, the following data were
obtained: cmpd A, 0.72 absorbance; B, 0.55; C, 0.80. For the unknown mixture, the data obtined when run under identical
conditions were as follows: cmpd A, .0.15 absorbance, B, 0.84; C, 0.75.
a) Calculate the relative percentage composition of the unknown mixture.
b) The distances traveled by the three components were 8.5, 6.3, and 5.5 cm, respectively.
Sketch the appearance of the paper chromatogram. Label each spot.
c) Calculate the Rf values if the solvent front is 18 cm.
Transcribed Image Text:A mixture of three components is separated using paper chromatography, and each component is analyzed using absorption. With standards containing 100 ppm of each component run simultaneously, the following data were obtained: cmpd A, 0.72 absorbance; B, 0.55; C, 0.80. For the unknown mixture, the data obtined when run under identical conditions were as follows: cmpd A, .0.15 absorbance, B, 0.84; C, 0.75. a) Calculate the relative percentage composition of the unknown mixture. b) The distances traveled by the three components were 8.5, 6.3, and 5.5 cm, respectively. Sketch the appearance of the paper chromatogram. Label each spot. c) Calculate the Rf values if the solvent front is 18 cm.
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