Answered: i) Determine the adjusted retention…

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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(a) An analyst has conducted a separation of a mixture of substance X and Y using liquid chromatography technique. The column used is 30-cm long and the flow rate was 0.65 mL/min. The chromatogram obtained is shown in Figure Q1.1. i) Determine the adjusted retention times for substance x and y. ii) Calculate the retention factors for substance x and y. iii) Calculate the resolution of the two peaks. Then, describe the quality of separation between the two adjacent peaks. iv) Calculate the plate height value of the column.

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Figure Q1.1: Chromatogram of the mixture X and Y substances
Detector response

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