i) Determine the adjusted retention times for substance x and y. ii) Calculate the retention factors for substance x and y. iii) Calculate the resolution of the two peaks. Then, describe the quality of separation between the two adjacent peaks.
i) Determine the adjusted retention times for substance x and y. ii) Calculate the retention factors for substance x and y. iii) Calculate the resolution of the two peaks. Then, describe the quality of separation between the two adjacent peaks.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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(a) An analyst has conducted a separation of a mixture of substance X and Y using liquid chromatography technique. The column used is 30-cm long and the flow rate was 0.65 mL/min. The chromatogram obtained is shown in Figure Q1.1.
i) Determine the adjusted retention times for substance x and y.
ii) Calculate the retention factors for substance x and y.
iii) Calculate the resolution of the two peaks. Then, describe the quality of
separation between the two adjacent peaks.
iv) Calculate the plate height value of the column.
Transcribed Image Text:16
14E
12
10
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70
Time, min
Figure Q1.1: Chromatogram of the mixture X and Y substances
Detector response
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