Which of the following statement about chromatography is CORRECT? In ion-exchange chromatography, charged substances are separated via column materials that carry a same charge. Using gradient elution in LC can shorten the separation time without sacrificing the resolution of early peaks. In normal-phase chromatography, if the order of solute polarities is A>B>C, then the order of retention time will be C>B>A (i.e. A will be eluted out first). In size-exclusion HPLC, larger molecules will be eluted out slower than smaller molecules.

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Chapter1: Chemical Foundations
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Which of the following statement about chromatography is CORRECT?
In ion-exchange chromatography, charged substances are separated via column materials that
carry a same charge.
Using gradient elution in LC can shorten the separation time without sacrificing the resolution of
early peaks.
In normal-phase chromatography, if the order of solute polarities is A>B>C, then the order of
retention time will be C>B>A (i.e. A will be eluted out first).
In size-exclusion HPLC, larger molecules will be eluted out slower than smaller molecules.
Transcribed Image Text:Which of the following statement about chromatography is CORRECT? In ion-exchange chromatography, charged substances are separated via column materials that carry a same charge. Using gradient elution in LC can shorten the separation time without sacrificing the resolution of early peaks. In normal-phase chromatography, if the order of solute polarities is A>B>C, then the order of retention time will be C>B>A (i.e. A will be eluted out first). In size-exclusion HPLC, larger molecules will be eluted out slower than smaller molecules.
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