Which of the following statement about chromatography is CORRECT? In ion-exchange chromatography, charged substances are separated via column materials that carry a same charge. Using gradient elution in LC can shorten the separation time without sacrificing the resolution of early peaks. In normal-phase chromatography, if the order of solute polarities is A>B>C, then the order of retention time will be C>B>A (i.e. A will be eluted out first). In size-exclusion HPLC, larger molecules will be eluted out slower than smaller molecules.
Which of the following statement about chromatography is CORRECT? In ion-exchange chromatography, charged substances are separated via column materials that carry a same charge. Using gradient elution in LC can shorten the separation time without sacrificing the resolution of early peaks. In normal-phase chromatography, if the order of solute polarities is A>B>C, then the order of retention time will be C>B>A (i.e. A will be eluted out first). In size-exclusion HPLC, larger molecules will be eluted out slower than smaller molecules.
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter26: An Introduction To Chromatographic Separations
Section: Chapter Questions
Problem 26.14QAP
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![Which of the following statement about chromatography is CORRECT?
In ion-exchange chromatography, charged substances are separated via column materials that
carry a same charge.
Using gradient elution in LC can shorten the separation time without sacrificing the resolution of
early peaks.
In normal-phase chromatography, if the order of solute polarities is A>B>C, then the order of
retention time will be C>B>A (i.e. A will be eluted out first).
In size-exclusion HPLC, larger molecules will be eluted out slower than smaller molecules.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fd60e293e-d4d8-4c11-baea-c1fc514e5dcb%2Fe74d03d1-f3d2-4070-ac6e-f3d8ba4ae879%2Fz6z36v2_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Which of the following statement about chromatography is CORRECT?
In ion-exchange chromatography, charged substances are separated via column materials that
carry a same charge.
Using gradient elution in LC can shorten the separation time without sacrificing the resolution of
early peaks.
In normal-phase chromatography, if the order of solute polarities is A>B>C, then the order of
retention time will be C>B>A (i.e. A will be eluted out first).
In size-exclusion HPLC, larger molecules will be eluted out slower than smaller molecules.
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