A fruit fly that is heterozygous for the autosomal recessive mutations black (b) body, purple (pu) eye color, and dumpy (dp) wing was test crossed to a fly that was homozygous for black body, purple eye and dumpy wings. The following progeny were produced.

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A fruit fly that is heterozygous for the autosomal recessive mutations black (b) body,
purple (pu) eye color, and dumpy (dp) wing was test crossed to a fly that was
homozygous for black body, purple eye and dumpy wings. The following progeny were
produced.
Wild type
Purple
Dumpy
Black
207
10
378
30
Purple, dumpy
Black, purple
Dumpy, black
Black, purple, dumpy
31
375
11
208
1250
A. What are the genotypes of the flies in the F1 test cross?
B. Which phenotypes represent the NCO classes?
C. Which phenotypes represent the DCO classes?
What are the genotypes of the flies in the F1 test cross?
dp + +/+ b pu X dp b pu/dp b pu
O dp + +/+ b pu X dp + +/+ b pu
O ++ +/dp b pu X dp b pu/dp b pu
O +b +/dp + pu X dp b pu/dp b pu
O +b +/dp + pu X dp + +/+ b pu
Which phenotypes represent the DCO classes?
Dumpy and black, purple
Wild-type and dumpy, black, purple
Purple and black, dumpy
Purple, black and dumpy
Which phenotypes represent the NCO classes?
O Dumpy and black, purple
Wild-type and dumpy, black, purple
Purple and black, dumpy
O Black, purple and dumpy
Transcribed Image Text:A fruit fly that is heterozygous for the autosomal recessive mutations black (b) body, purple (pu) eye color, and dumpy (dp) wing was test crossed to a fly that was homozygous for black body, purple eye and dumpy wings. The following progeny were produced. Wild type Purple Dumpy Black 207 10 378 30 Purple, dumpy Black, purple Dumpy, black Black, purple, dumpy 31 375 11 208 1250 A. What are the genotypes of the flies in the F1 test cross? B. Which phenotypes represent the NCO classes? C. Which phenotypes represent the DCO classes? What are the genotypes of the flies in the F1 test cross? dp + +/+ b pu X dp b pu/dp b pu O dp + +/+ b pu X dp + +/+ b pu O ++ +/dp b pu X dp b pu/dp b pu O +b +/dp + pu X dp b pu/dp b pu O +b +/dp + pu X dp + +/+ b pu Which phenotypes represent the DCO classes? Dumpy and black, purple Wild-type and dumpy, black, purple Purple and black, dumpy Purple, black and dumpy Which phenotypes represent the NCO classes? O Dumpy and black, purple Wild-type and dumpy, black, purple Purple and black, dumpy O Black, purple and dumpy
Expert Solution
Step 1

Formulas for three point test cross -

A. Distance between two genes = (#progenies obtained by SCO between those two genes + #progenies obtained by DCO) / total #progenies × 100

B. Expected DCO = product of distances between first two and next two genes

C. Coefficient of coincidence = observed DCO/expected DCO

D. Interference = 1 - coc

E. Determine gene order by comparing DCO and parental progenies

F. Parental progenies = maximum in #

G. DCO progenies = minimum in # 

 

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