A dinical test sometimes used to diagnose folate deficiency or B,2 deficiencyis a histidine tolerance test, where one injects a large dose of histidine intothe bloodstream and then carries out a series of biochemical determinations.What histidine metabolite would you expect to accumulate in a folate- orB12-deficient patient, and why?

Folic acid, vitamin B12, and vitamin C are responsible for the metabolism of proteins. Folate is…

Answered: A dinical test sometimes used to…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

the half life of metformin ( Glucophage) is 17.6 hours. What is the rate of decay?
G418 disulfate (FW 692.7) is an antibiotic used to select transfected mammalian cells in tissue culture. The standard working concentration for selection is 400 µg/mL of active ingredient in culture medium. The bottle states that it contains 71% active ingredient. You need to make a 100x stock in medium and adjust the pH before you apply it to cells. How much powder should be added to make 10 mL of stock solution? How much do you add to a 2 mL culture dish?
Despite the fact that many peptides have critical physiological functions(e.g., as hormones, neurotransmitters, antibacterial toxins), they are not considered to be ideal as drugs. The following questions illustrate some of the issues that limit the use of peptides as therapeutics.(a) Insulin is a peptide therapeutic used to manage Type 1 diabetes, whichaffects more than 20 million people worldwide according to the International Diabetes Federation. A significant limitation to the broad distribution and use of insulin to treat Type 1 diabetes is the fact that it must be administered by injection rather than orally. Why is insulin administered by injection and not orally?(b) Many of the D-amino acids found in nature have been discovered inbacterially produced peptides that have antibiotic properties. Bacteriasecrete these peptides into their environments to kill competitor bacteriaand thereby gain a selective advantage. Given your answer to part (a) ofthis question, what potential…
During successful purification of every enzyme, the following may be expected: Select ALL that apply. 1. Solubility in NaCl increases 2. The activity increases 3. The specificity increases 4. The number of subunits increases 5. the epitope number increases
Which of the following is incorrect about blood glucose test interpretation A) fasting plasma glucose 6.1-6.8 defined as having IGT and should probably be screened again after a year or so. B) 2 results>7mmmol/l defined as having diabetes C) fasting plasma glucose >6mmol, recall for further test. D) fasting or random plasma glucose, on one reading of <11.1mmol/l is diagnostic of diabetes
What is the chemistry behind of the effect of proteins when it is subjected to different temperatures done in three trials? see the data below to interpret Table 3.1 %Transmittance of Identical 10% albumin samples subjected to different temperatures done in three trials. Heating Tempt. (C) %Transmittance Trial 1 Trial 2 trial 3 Average Control (unheated) 62 62 62 62 55 60 59 58 59 60 2.5 4.5 4.5 11.5 65 1.52 1.6 1.52 1.55 70 1 1 1 1 Table 3.2 Absorbance of Identical 10% albumin solution subjected to different temperatures done in three trials. Heating Tempt. (C) Absorbance Readings (450nm) Trial 1 Trial 2 Trial 3 Average Control (unheated) 0.2076 0.2076 0.2076 0.2076 55 0.2218 0.2219 0.2366 0.2292 60 1.6021 1.3468 1.3468 0.9393 65 1.8182 1.7959 1.8182 1.897 70 2 2 2 2
There are several applications of microorganisms in industries. One of them is in medical and pharmaceuticals. Name a suitable microorganism and demonstrate its application in producing synthetic human insulin.
Upon doing the experiment of Protein Denaturation, what could happen in the precipitation of strong acids if small amount of water is added to albumin solution added to a test tube and added to another tube containing concentrated nitric acid? Explain clearly.
A lipase enzyme has been isolated from a bacterium with a strong red color, found in industrial waste from a fishing company. This enzyme has great potential for elaboration of degreasers for industrial use as it can work at a wide range of temperature (between 4 ° and 80 ° C). However, it is estimated that its purity for industrial purposes is only 35% since it is contaminated with a low molecular weight protein that binds the smallest red pigment peptides and salts product of the purification process 1- Propose a methodology to increase the degree of purity of this enzyme, eliminatingsmall proteins and color.2- How it is ensured that the degree of purity of the enzyme has increased.
Various concentrations of recombinant human insulin were prepared for use standards for an HPLC method. To verify the prepared concentrations, the samples were analyzed by measuring the absorbance at 280 nm in a short path length (5 mm) cuvette. The molar absorption coefficient for human insulin is approximately 5.875 x 10³ M-¹cm-¹. a. Calculate the extinction coefficient in mL mg-¹cm-¹. b. Calculate the concentrations of the following human insulin standards if the measured absorbances and dilutions used are: Standard 1 Standard 2 Abs. (at 280 nm) of Diluted Sample 0.305 0.685 Dilution 145.0 µL sample, 25.0 μL buffer 130.0 µL sample, 40.0 μL buffer
A cell with an internal concentration of 0.03 molar glucose is placed in a test tube containing 0.02 molar glucose solution. Assuming that glucose is not actively transported into the cell, describe the internal concentration of the cell relative to its environment?
Which of the following would result in an increase in the amount of F-2,6-BP? (select all that apply) Group of answer choices high blood glucose activation of fructose bisphosphatase 2 increased phosphofructokinase 2 activity increased glucagon secretion increased phosphorylation of the bifunctional enzyme

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