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a. What is the optimum pH of wild type β-galactosidase?         _________ b. What is the optimum temperature of mutant β-galactosidase?         _________ c. Which enzyme has the greater activity at pH 7.2? _________ d. Which enzyme has the greater activity at a temperature of 42.5oC? _________ e. Which enzyme has greater activity if pH decreases from 7.5 to 6.4?            _________ f. Which enzyme has greater activity if temperature increases from 40oC to 41 oC

a. What is the optimum pH of wild type β-galactosidase?         _________ b. What is the optimum temperature of mutant β-galactosidase?         _________ c. Which enzyme has the greater activity at pH 7.2? _________ d. Which enzyme has the greater activity at a temperature of 42.5oC? _________ e. Which enzyme has greater activity if pH decreases from 7.5 to 6.4?            _________ f. Which enzyme has greater activity if temperature increases from 40oC to 41 oC

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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  1. Refer to the figure below and answer the following questions:

Legend: Blue – wild-type β-galactosidase; Red – mutant β-galactosidase

 

_________ a. What is the optimum pH of wild type β-galactosidase?        

_________ b. What is the optimum temperature of mutant β-galactosidase?        

_________ c. Which enzyme has the greater activity at pH 7.2?

_________ d. Which enzyme has the greater activity at a temperature of 42.5oC?

_________ e. Which enzyme has greater activity if pH decreases from 7.5 to 6.4?           

_________ f. Which enzyme has greater activity if temperature increases from 40oC to 41 oC?   

A 45 55 65 75 B. 13 Temperature (C) - -gNactosidane acivity IE Uiml
Transcribed Image Text:A 45 55 65 75 B. 13 Temperature (C) - -gNactosidane acivity IE Uiml
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