A constitutive mutant is a strain that continuously makes aprotein that is inducible in the wild type. Describe two waysin which a change in a DNA molecule could lead to theemergence of a constitutive mutant. How could these twotypes of constitutive mutants be distinguished genetically?

Q: T. Miyake and M. Demerec examined proline-requiring mutations in the bacterium Salmonella…

A: In transduction process, the closer the genes are present on a DNA, the more possibility they have…

Q: BACTERIA STRAIN A IS AUXOTROPHIC FOR METHIONINE AND STRAIN B IS AUXOTROPHIC FOR LEUCINE. A. WILL…

A: Auxotrophic strains lack the property of synthesising the growth factors required for their proper…

Q: To detect the CAG repeat expansion with a particular gene where 30 repeats in Normal changes to 250…

A: Hi! Thank you for the questions. As you have posted multiple questions, I will be answering the…

Q: The following shows the genotype of a partial diploid bacterial cell - where one chromosomal region…

A: The lactose operon is a cluster of genes present in E. coli and other bacteria that are specifically…

Q: Which step of the Central Dogma is responsible for transmission of genetic information from…

A: The central dogma is a process through which the information present in the DNA is converted into…

Q: Transgenic tobacco plants were obtained in which the vector Ti plasmid was designed to insert the…

A: A gene is a unit of hereditary present in thousands of numbers on the helical strands of…

Q: A cystic-fibrosis mutation in a certain pedigree is due toa single nucleotide-pair change. This…

A: To explain: To explain about careers of cystic fibrosis and the risks of having cystic fibrosis…

Q: V/D/J recombination frequently yields a gene whose mRNA cannot be successfully translated into an…

A: Somatic recombination is the process for generating antibody and T-cell diversity in mammalian…

Q: How does homologous recombination with transfected disruption constructs can inactivate specific…

A: The plasmid does not contain the yeast origin of replication. The gene target constructed to…

Q: An Hfr strain that is hisE + and pheA + was conjugated to a strain that is hisE − and pheA −. The…

A: Conjugation experiments are done to map different genes in bacteria. The mapped distances determined…

Q: Is homologous recombination an example of mutation? Explain.

A: Mutations are the changes in the DNA or chromosome. From changes in one base to the small fragment…

Q: design an experiment to use these DNA sequences to distinguish between these two hypotheses.

A: Ans) Before designing this experiment we need to know the hypothesis given by Mendel who had done…

Q: Can you suggest how we could have determined whether Bill’s aunts were carriers of the mutant BTK…

A: The Bruton tyrosine kinase is important for the growth and maturation of B cells by some change and…

Q: Knockout mice are mice in which a functional gene or a group of functional genes are rendered…

A: What happened when RAG1 and RAG2 genes removed to make knockout mice?

Q: After isolating six Neurospora mutations, you mate A and a representatives of each in all pairwise…

A: We can determine that there are at least 4 different genes represented in this experiment, with the…

Q: Given a mutation in the promoter, coding region, and/or non-coding region of a gene, explain how…

A: The mutation is a sudden heritable change in the genetic makeup of a cell which mostly results in…

Q: A mutant has no activity for the enzyme isocitrate lyase.Does this result prove that the mutation is…

A: Isocitrate lyase is an enzyme in the glycoxylate cycle that catalyzes the cleavage of isocitrate to…

Q: A yeast geneticist irradiates haploid cells of a strain thatis an adenine-requiring auxotrophic…

A: A mutant strain that isn't able to grow on a minimal medium and requires some specific supplements…

Q: An Hfr strain that is hisE+ and pheA+ was mixed with a strain thatis hisE− and pheA−. The…

A: Transfer of genes in prokaryotes occurs through horizontal transfer mechanisms such as transduction,…

Q: Hydroxylamine (HA) causes only G • C → A • T transitions in DNA. Will HA produce nonsense mutations…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: 3) You have identified an interesting mutant in gene P. Using a Punnett square, demonstrate the…

A: The pure breeding lines of mutant and wild type organism must be crossed to determine if the…

Q: A normal appearing female infant was identified with a positive newborn screen, linked to chromosome…

A: Hereditary genetic disorders can be devastating for the family and these are screened as early as…

Q: Not all inherited traits are determined by nuclear genes (i.e., genes located in the cell nucleus)…

A: The process of producing a genetically identical clone of an animal is called as reproductive…

Q: Which of the following statements about inherited DNA repair syndromes are correct (select all that…

A: DNA repair deficiency disorder:  Generally when DNA undergoes any sort of DNA damage these damages…

Q: A fluctuation test was carried out to determine the rate of mutation to Azetidine resistance (a…

A: The inventors of fluctuation test were Delburk and Luria in 1943. This test aimed to determine…

Q: Given the following complementation chart for holes in Monstera, give me the biochemical (phenotype)…

A: When the normal phenotype is restored by the complementation effect of the two mutants, then it is…

Q: Brenner’s m mutant phages (m1–m6) described inFig. 8.8 were suppressed when grown in suppressor(su−)…

A: Transfer ribonucleic acid (tRNA) is a type of RNA molecule that helps in decoding a messenger RNA…

Q: Homologous recombination in E. coli forms heteroduplex regions of DNA containing mismatched bases.…

A: MMR of Mismatch Repair system of DNA is referred to as a system for identifying and repairing the…

Q: An Hfr strain that is hisE+ and pheA+ was mixed with a strain thatis hisE− and pheA−. The…

A: Transfer of genes in prokaryotes occurs through horizontal transfer mechanisms such as transduction,…

Q: E. coli cells are simultaneously infected with two strains of phage λ. One strain has a mutant host…

A: A gene is a stretch of nucleotides present in the DNA molecule. It encodes information for the…

Q: You have identified a SNP marker that in one largefamily shows no recombination with the locus…

A: An SNP (Single nucleotide polymorphism) marker has been identified that shows no recombination…

Q: In cells, certain hormones, such as epinephrine, have the ability to raise the concentration of…

A: The ability of a protein to bind to a DNA segment can be analyzed using the method of…

Q: 13) What is the purpose of the negative selectable marker in a mouse knock out cassette?  a) Why…

A: The negative selection marker used for the creation of knock-out mouse is thymidine kinase gene…

Q: Mutants tested 1,2 1,3 1,4 1,5 Which would be a possible arrangement of mutations on the two rll…

A: A complementation test serves as a genetic experiment designed to assess if two independently…

Q: You have isolated 8 mutants in yeast that fail to grow on minimal media plates but do grow when they…

A: This is a question from classical genetics. Here one mutant strain is crossed with other mutant to…

Q: In the transformation experiment by Griffith, two strains of an organism was injected into a mouse…

A: Frederick Griffith was the man who performed the transformation principle on Streptococcus…

Q: T. Miyake and M. Demerec examined proline-requiring mutations in the bacterium Salmonella…

A: Transduction is the process by which a virus transfers genetic material from one bacterium to…

Mitochondrial Mutations

A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.