A bacterial enzyme catalyzes the hydrolysis of maltose as shown in the reaction given below:   Maltose + H2O  -> 2 glucose If the reaction has a Km of 0.135 mM and a V max of 65 m mol/min. What is the reaction velocity when the concentration of maltose is 1.0 mM?

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Q: [S] mM 20 50 100 200 300 500 700 Initial Velocity (mM/min) 1.012 1.950 2.823 3.637 4.024 4.398 4.580

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Q: Km V. (V) 15) + V = [S] max a' = 2 a' = 1.5 (7) -15⁰° max a Vmax 1 [S] MM a'=1 [1]

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Q: L(24 points) Explain How is the Michaelis constant defined. and what does a low or high value for Km…

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Q: Calculate the Km of the above enzyme.

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Q: Calculate the equilibrium concentration of H2O for the following esterification reaction performed…

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Q: Vo with Vo without [Substrate, µM] DEDS DEDS (µM/min) (µM/min) 3.333 0.774 1.196

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