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8. Which reporter construct design would be best for moni IGFP green when, and how much a gene-of-interest is transcribed? fluorescent protein) A. Replace the promoter with the GFP sequence, leaving the CRM and gene coding region intact. B. Include the CRM and promoter but replace the gene coding region with the GFP sequence. C. Include the CRM and promoter and add the GFP sequence to the end of the gene coding region, in the same reading frame. D. Replace the CRM with the GFP sequence and leave the promoter and gene coding region intact.

8. Which reporter construct design would be best for moni IGFP green when, and how much a gene-of-interest is transcribed? fluorescent protein) A. Replace the promoter with the GFP sequence, leaving the CRM and gene coding region intact. B. Include the CRM and promoter but replace the gene coding region with the GFP sequence. C. Include the CRM and promoter and add the GFP sequence to the end of the gene coding region, in the same reading frame. D. Replace the CRM with the GFP sequence and leave the promoter and gene coding region intact.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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### Genetic and Molecular Biology Assessment #### 8. Monitoring Gene Transcription Using Reporter Constructs **Question:** Which reporter construct design would be best for monitoring where, when, and how much a gene-of-interest is transcribed? (GFP = green fluorescent protein) **Options:** A. Replace the promoter with the GFP sequence, leaving the CRM and gene coding region intact. B. Include the CRM and promoter but replace the gene coding region with the GFP sequence. C. Include the CRM and promoter and add the GFP sequence to the end of the gene coding region, in the same reading frame. D. Replace the CRM with the GFP sequence and leave the promoter and gene coding region intact. --- #### 9. Insertion Sequence Elements and Mutations **Question:** Which feature, if mutated, would destroy the ability of an insertion sequence element to be transposed? **Options:** A. Its transposase gene B. Its reverse transcriptase gene C. Its target site duplication D. Its inverted repeats --- #### 10. Understanding Poly-A Tails **Question:** What is a poly-A tail? **Options:** A. The region where the ribosome initiates translation B. The termination signal of bacterial translation C. The complementary base-pairing region that forms stem-loop structures D. A modification of the 3’ end of eukaryotic transcripts --- #### 11. Placental Cells and ERVs **Question:** Why can placental cells "afford" to express ERVs, while they are silenced in differentiating cells during early development? **Options:** A. The placenta is a transient organ, so fitness costs are unlikely to be high B. ERVs stimulate the baby's immune system C. Placental cells arise from eggs, which have most of the cytoplasm D. Placental cells generate more ATP, so replication of extra DNA is not a problem --- For additional educational resources on genetics and molecular biology, please explore our [Resource Library](#). If you have any queries or need further assistance, don't hesitate to contact our Academic Support Team.
Transcribed Image Text:### Genetic and Molecular Biology Assessment #### 8. Monitoring Gene Transcription Using Reporter Constructs **Question:** Which reporter construct design would be best for monitoring where, when, and how much a gene-of-interest is transcribed? (GFP = green fluorescent protein) **Options:** A. Replace the promoter with the GFP sequence, leaving the CRM and gene coding region intact. B. Include the CRM and promoter but replace the gene coding region with the GFP sequence. C. Include the CRM and promoter and add the GFP sequence to the end of the gene coding region, in the same reading frame. D. Replace the CRM with the GFP sequence and leave the promoter and gene coding region intact. --- #### 9. Insertion Sequence Elements and Mutations **Question:** Which feature, if mutated, would destroy the ability of an insertion sequence element to be transposed? **Options:** A. Its transposase gene B. Its reverse transcriptase gene C. Its target site duplication D. Its inverted repeats --- #### 10. Understanding Poly-A Tails **Question:** What is a poly-A tail? **Options:** A. The region where the ribosome initiates translation B. The termination signal of bacterial translation C. The complementary base-pairing region that forms stem-loop structures D. A modification of the 3’ end of eukaryotic transcripts --- #### 11. Placental Cells and ERVs **Question:** Why can placental cells "afford" to express ERVs, while they are silenced in differentiating cells during early development? **Options:** A. The placenta is a transient organ, so fitness costs are unlikely to be high B. ERVs stimulate the baby's immune system C. Placental cells arise from eggs, which have most of the cytoplasm D. Placental cells generate more ATP, so replication of extra DNA is not a problem --- For additional educational resources on genetics and molecular biology, please explore our [Resource Library](#). If you have any queries or need further assistance, don't hesitate to contact our Academic Support Team.
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