6.1 EXPECTED RESULT FOR CELL COUNT USING HAEMOCYTOMETER Table 2 Number of cell in upper grid Grid location number Number of cell in lower grid 20 17 1 15 3 13 4 10 14 18 13 7 20 17 15 13 10 14 10 12 13 18 Total cells in upper and lower grid 120 120 TOTAL CELLS 240 Total grids 16 Please calculate a numbers of cell/ml from the information table 2.

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6.1 EXPECTED RESULT FOR CELL COUNT USING HAEMOCYTOMETER
Table 2
Number of cell in upper
grid
15
13
Grid location number
Number of cell in lower
grid
20
17
1
3
4
10
14
18
13
7
20
15
17
14
13
10
10
12
13
18
Total cells in upper
and lower grid
120
120
TOTAL CELLS
240
Total grids
16
Please calculate a numbers of cell/ml from the information table 2.
Transcribed Image Text:6.1 EXPECTED RESULT FOR CELL COUNT USING HAEMOCYTOMETER Table 2 Number of cell in upper grid 15 13 Grid location number Number of cell in lower grid 20 17 1 3 4 10 14 18 13 7 20 15 17 14 13 10 10 12 13 18 Total cells in upper and lower grid 120 120 TOTAL CELLS 240 Total grids 16 Please calculate a numbers of cell/ml from the information table 2.
Cell counting calculation
Basic hemacytometer formula
Neubauer Hemacytometer
Calculation
Number of cells counted X dilution factor
total volume of the cells counted
total volume = # primary squares x 0.1 (depth)
Cells X dilution = cells/uL
squares X 0.1
This formula can be used with any counting
chamber as long as one knows the
dimensions for the chamber
Note: 0.1 mm depth is constant in Neubauer Hemacytometer
Size
Volume
Blue
0.0025 mm?
0.25 nl
Yellow
0.04 mm?
4 nl
Green
0.0625 mm?
6.25 nl
Red
1 mm?
100 nl
average live cells × dilution factor
volume of a square (mL)
Cell density (cells/mL) =
Transcribed Image Text:Cell counting calculation Basic hemacytometer formula Neubauer Hemacytometer Calculation Number of cells counted X dilution factor total volume of the cells counted total volume = # primary squares x 0.1 (depth) Cells X dilution = cells/uL squares X 0.1 This formula can be used with any counting chamber as long as one knows the dimensions for the chamber Note: 0.1 mm depth is constant in Neubauer Hemacytometer Size Volume Blue 0.0025 mm? 0.25 nl Yellow 0.04 mm? 4 nl Green 0.0625 mm? 6.25 nl Red 1 mm? 100 nl average live cells × dilution factor volume of a square (mL) Cell density (cells/mL) =
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