4-19. In a common protein analysis, a dye binds to the protein and the color of the dye changes from brown to blue. The intensity of blue color is proportional to the amount of protein present. Protein (µg): 0.00 9.36 18.72 28.08 37.44 Absorbance: 0.466 0.676 0.883 1.086 1.280 (a) After subtracting the blank absorbance (0.466) from the remaining absorbances, use the method of least squares to determine the equation of the best straight line through these five points (n and intercept to express the equation in the form y (±s,) = [m (±sm)]x + [b (±sµ)] with a reasonable number of signifi- cant figures. 5). Use the standard deviation of the slope (b) Make a graph showing the experimental data and the cal- culated straight line. (c) An unknown gave an observed absorbance of 0.973. Cal- culate the number of micrograms of protein in the unknown, and estimate its uncertainty,
4-19. In a common protein analysis, a dye binds to the protein and the color of the dye changes from brown to blue. The intensity of blue color is proportional to the amount of protein present. Protein (µg): 0.00 9.36 18.72 28.08 37.44 Absorbance: 0.466 0.676 0.883 1.086 1.280 (a) After subtracting the blank absorbance (0.466) from the remaining absorbances, use the method of least squares to determine the equation of the best straight line through these five points (n and intercept to express the equation in the form y (±s,) = [m (±sm)]x + [b (±sµ)] with a reasonable number of signifi- cant figures. 5). Use the standard deviation of the slope (b) Make a graph showing the experimental data and the cal- culated straight line. (c) An unknown gave an observed absorbance of 0.973. Cal- culate the number of micrograms of protein in the unknown, and estimate its uncertainty,
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
![t, a
The
n of
4-19.
In a common protein analysis, a dye binds to the
protein and the color of the dye changes from brown to blue.
The intensity of blue color is proportional to the amount of
protein present.
Protein (ug):
0.00
9.36
18.72
28.08
37.44
Absorbance:
0.466
0.676
0.883
1.086
1.280
2
(a) After subtracting the blank absorbance (0.466) from the
remaining absorbances, use the method of least squares to
determine the equation of the best straight line through these
five points (n
and intercept to express the equation in the form y (±s,) =
[m (±sm)]x + [b (±sp)] with a reasonable number of signifi-
cant figures.
02
5). Use the standard deviation of the slope
and
and
(b) Make a graph showing the experimental data and the cal-
culated straight line.
and
(c) An unknown gave an observed absorbance of 0.973. Cal-
culate the number of micrograms of protein in the unknown,
and estimate its uncertainty.
nree](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fd828d166-d9fc-425f-a3e7-88dc2f24978c%2Ffc127fd1-ffc0-481d-a6c4-d54030c1799a%2Fk08hy0u_processed.jpeg&w=3840&q=75)
Transcribed Image Text:t, a
The
n of
4-19.
In a common protein analysis, a dye binds to the
protein and the color of the dye changes from brown to blue.
The intensity of blue color is proportional to the amount of
protein present.
Protein (ug):
0.00
9.36
18.72
28.08
37.44
Absorbance:
0.466
0.676
0.883
1.086
1.280
2
(a) After subtracting the blank absorbance (0.466) from the
remaining absorbances, use the method of least squares to
determine the equation of the best straight line through these
five points (n
and intercept to express the equation in the form y (±s,) =
[m (±sm)]x + [b (±sp)] with a reasonable number of signifi-
cant figures.
02
5). Use the standard deviation of the slope
and
and
(b) Make a graph showing the experimental data and the cal-
culated straight line.
and
(c) An unknown gave an observed absorbance of 0.973. Cal-
culate the number of micrograms of protein in the unknown,
and estimate its uncertainty.
nree
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