2.1. For an enzyme (5µM), the following initial velocities have been reported depending on the substrate concentration: [Substrate], mM Vo 0.02 10.83 0.04 18.57 0.07 26.76 0.1 32.50 0.15 39.00 0.2 43.33 0.3 48.75 0.5 54.17 0.7 56.88 2.2. Sketch the Eadie-Hofstee plot for the enzyme reaction 2.3. Determine Km and Vmax for this enzyme 2.4. Indicate where Vmax and Km can be recognized

Chemistry: The Molecular Science
5th Edition
ISBN:9781285199047
Author:John W. Moore, Conrad L. Stanitski
Publisher:John W. Moore, Conrad L. Stanitski
Chapter11: Chemical Kinetics: Rates Of Reactions
Section: Chapter Questions
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2.1. For an enzyme (5µM), the following initial velocities have been reported depending
on the substrate concentration:
[Substrate], mM Vo
0.02
10.83
0.04
18.57
0.07
26.76
0.1
32.50
0.15
39.00
0.2
43.33
0.3
48.75
0.5
54.17
0.7
56.88
2.2. Sketch the Eadie-Hofstee plot for the enzyme reaction
2.3. Determine Km and Vmax for this enzyme
2.4. Indicate where Vmax and Km can be recognized
Transcribed Image Text:2.1. For an enzyme (5µM), the following initial velocities have been reported depending on the substrate concentration: [Substrate], mM Vo 0.02 10.83 0.04 18.57 0.07 26.76 0.1 32.50 0.15 39.00 0.2 43.33 0.3 48.75 0.5 54.17 0.7 56.88 2.2. Sketch the Eadie-Hofstee plot for the enzyme reaction 2.3. Determine Km and Vmax for this enzyme 2.4. Indicate where Vmax and Km can be recognized
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