2.1. For an enzyme (5µM), the following initial velocities have been reported depending on the substrate concentration: [Substrate], mM Vo 0.02 10.83 0.04 18.57 0.07 26.76 0.1 32.50 0.15 39.00 0.2 43.33 0.3 48.75 0.5 54.17 0.7 56.88 2.2. Sketch the Eadie-Hofstee plot for the enzyme reaction 2.3. Determine Km and Vmax for this enzyme 2.4. Indicate where Vmax and Km can be recognized

2.1. For an enzyme (5µM), the following initial velocities have been reported depending on the substrate concentration: [Substrate], mM Vo 0.02 10.83 0.04 18.57 0.07 26.76 0.1 32.50 0.15 39.00 0.2 43.33 0.3 48.75 0.5 54.17 0.7 56.88 2.2. Sketch the Eadie-Hofstee plot for the enzyme reaction 2.3. Determine Km and Vmax for this enzyme 2.4. Indicate where Vmax and Km can be recognized

Chemistry: The Molecular Science

5th Edition

ISBN:9781285199047

Author:John W. Moore, Conrad L. Stanitski

Publisher:John W. Moore, Conrad L. Stanitski

Chapter11: Chemical Kinetics: Rates Of Reactions

Section: Chapter Questions

Problem 81QRT

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