SPECIFY THE FUNCTIONS AND ENZYMES USED FOR PROTEIN DIGESTIONProcedure: Peptic digestionPrepare 4 test tubes as follows: Tube 1 - 3 ml pepsin Tube 2 - 3 ml of 0.4% HCl Tube 3 - 3 ml pepsin + 1.5 ml of 0.4% HCl Tube 4 - 3 ml pepsin + 1.5 ml of 1% Na2CO3Add equal slices of hard-boiled egg white to each tube.Cover and incubate at 40oC for 2 hours.Add 4 drops of toluene and store till the next lab period.Determine the extent of digestion by visually noting the size of the hard-boiled egg white in each tube.Withdraw 1 ml of the supernatant liquid from each tube and place in 4 separate test tubes labelled tube 1a. 2a, 3a, 4a.Neutralize tubes 2a& 3a with solid sodium carbonate.To each supernatant liquid, add 1 ml of 10% NaOH and 2 drops of 1% CuSO4. Compare the colors obtained in the 4 tubes.Place 1 ml of 0.5% peptone in a test tube and add 1 ml of 10% NaOH and 2 drops of 1% CuSO4. Compare the result of these tubes with the other 4 tubes above. Pancreatin digestionPrepare 4 test tubes as follows: Tube 1 - 3 ml neutral pancreatin Tube 2 - 3 ml water Tube 3 - 3 ml water + 1.5 ml pancreatin Tube 4 - 3 ml water + 1.5 ml pancreatin + 1.5 ml 1% Na2CO3Place equal size of hard-boiled egg white in all tubes.Place all tubes in water bath with a temperature maintained at 40oC for 1.5 hours.Add 3 drops of toluene in all tubes and incubate till the next lab period.Observe the size of the egg white in each of the tubes.Take 1 ml of the supernatant in each tube and place in 4 separate test tubes labelled 1b,2b,3b,4b. Add 1 ml of 10% NaOH and 2 drops of 1% CuSO4 in each tube. Mix and compare the result of the 4 tubes. 2. What is the role of pepsin and pancreatin in protein digestion? Where can theseenzymes be found in the body?Role of pepsinPepsin is found in :Role of PancreatinPancreatin is found in :

Enzymes used for protein digestion are called proteases, Based on their cleavage site proteases can…

Answered: 2. What is the role of pepsin and…

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter47: Animal Nutrition

Section: Chapter Questions

Problem 7TYK: If the fat in whole milk is ingested: a. the stomach, with its high pH, will stimulate cells of the...

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Question

SPECIFY THE FUNCTIONS AND ENZYMES USED FOR PROTEIN DIGESTION

Procedure:

Tube 1 - 3 ml pepsin

Tube 2 - 3 ml of 0.4% HCl

Tube 3 - 3 ml pepsin + 1.5 ml of 0.4% HCl

Tube 4 - 3 ml pepsin + 1.5 ml of 1% Na₂CO₃

Add equal slices of hard-boiled egg white to each tube.
Cover and incubate at 40^oC for 2 hours.
Add 4 drops of toluene and store till the next lab period.
Determine the extent of digestion by visually noting the size of the hard-boiled egg white in each tube.
Withdraw 1 ml of the supernatant liquid from each tube and place in 4 separate test tubes labelled tube 1a. 2a, 3a, 4a.
Neutralize tubes 2a& 3a with solid sodium carbonate.
To each supernatant liquid, add 1 ml of 10% NaOH and 2 drops of 1% CuSO₄. Compare the colors obtained in the 4 tubes.
Place 1 ml of 0.5% peptone in a test tube and add 1 ml of 10% NaOH and 2 drops of 1% CuSO₄. Compare the result of these tubes with the other 4 tubes above.

Tube 1 - 3 ml neutral pancreatin

Tube 2 - 3 ml water

Tube 3 - 3 ml water + 1.5 ml pancreatin

Tube 4 - 3 ml water + 1.5 ml pancreatin + 1.5 ml 1% Na₂CO₃

Place equal size of hard-boiled egg white in all tubes.
Place all tubes in water bath with a temperature maintained at 40^oC for 1.5 hours.
Add 3 drops of toluene in all tubes and incubate till the next lab period.
Observe the size of the egg white in each of the tubes.
Take 1 ml of the supernatant in each tube and place in 4 separate test tubes labelled 1b,2b,3b,4b. Add 1 ml of 10% NaOH and 2 drops of 1% CuSO₄ in each tube. Mix and compare the result of the 4 tubes.