11- During the acid hydrolysis of a protein (6N HCl at 110°C for 24, 48 and 72 hours) to determine the amino acid composition: a- Serine and Threonine are completely destroyed at all time points and cannot be quantitated. b- Tryptophan is completely destroyed at all time points and cannot be quantitated. C- Asparagine and Glutamine are slowly converted to Aspartate and Glutamate and a time course correction must be used. d- Histidine is slowly destroyed and a time course correction must be used. e- Peptide bonds involving hydrophobic residues are slowly hydrolyzed and time course analysis must be used. Which of the above statements are true?
11- During the acid hydrolysis of a protein (6N HCl at 110°C for 24, 48 and 72 hours) to determine the amino acid composition: a- Serine and Threonine are completely destroyed at all time points and cannot be quantitated. b- Tryptophan is completely destroyed at all time points and cannot be quantitated. C- Asparagine and Glutamine are slowly converted to Aspartate and Glutamate and a time course correction must be used. d- Histidine is slowly destroyed and a time course correction must be used. e- Peptide bonds involving hydrophobic residues are slowly hydrolyzed and time course analysis must be used. Which of the above statements are true?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:11- During the acid hydrolysis of a protein (6N HCl at 110°C for 24, 48 and 72
hours) to determine the amino acid composition:
a- Serine and Threonine are completely destroyed at all time points and cannot
be quantitated.
b- Tryptophan is completely destroyed at all time points and cannot be
quantitated.
c- Asparagine and Glutamine are slowly converted to Aspartate and Glutamate
and a time course correction must be used.
d- Histidine is slowly destroyed and a time course correction must be used.
e- Peptide bonds involving hydrophobic residues are slowly hydrolyzed and
time course analysis must be used.
Which of the above statements are true?
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