1. Plate count data are used to calculate microbial population density as colony forming units (CFUs) per milliliter of culture. Culture density (CFU/mL) = number of colonies (dilution factor) x (volume plated, in mL) Use these data below to calculate the CFU/mL in this sample of bacteria after 1 hr of growth. **Important to know for these types of questions - only use colony counts that fall between 10 and 300. TNTC = too numerous to count, so many colonies that you can't distinguish them to count. Use the plates with numbers of colonies that can be counted, calculate the CFU on each one, and then calculate the average between all of them. Looking for more assistance? Read section 7.3 in the textbook. Time 1h Dilution (before plating) none 1:10 (1 x 10-¹) 1:100 (1 x 10-²) 1:1,000 (1 x 10-³) Volume spread on plate 0.1 mL 0.1 mL 0.1 mL 0.1 mL Number of colonies observed (replicate plates) TNTC 499 83 10 TNTC 518 87 8 TNTC indicates too numerous to count. *Cell density could not be calculated accurately because plates contained too many colonies to be counted, too many colonies to be counted accurately (>300), or too few colon to be meaningful (<10)

1. Plate count data are used to calculate microbial population density as colony forming units (CFUs) per milliliter of culture. Culture density (CFU/mL) = number of colonies (dilution factor) x (volume plated, in mL) Use these data below to calculate the CFU/mL in this sample of bacteria after 1 hr of growth. **Important to know for these types of questions - only use colony counts that fall between 10 and 300. TNTC = too numerous to count, so many colonies that you can't distinguish them to count. Use the plates with numbers of colonies that can be counted, calculate the CFU on each one, and then calculate the average between all of them. Looking for more assistance? Read section 7.3 in the textbook. Time 1h Dilution (before plating) none 1:10 (1 x 10-¹) 1:100 (1 x 10-²) 1:1,000 (1 x 10-³) Volume spread on plate 0.1 mL 0.1 mL 0.1 mL 0.1 mL Number of colonies observed (replicate plates) TNTC 499 83 10 TNTC 518 87 8 TNTC indicates too numerous to count. *Cell density could not be calculated accurately because plates contained too many colonies to be counted, too many colonies to be counted accurately (>300), or too few colon to be meaningful (<10)

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Which of the following is NOT correctly matched? A. Halophile – microbe that can grow in an environment with high osmotic pressure (e.g., salt) B. None of the other four answers (all are correctly matched) C. Agar – complex polysaccharide from seaweed used as a solidifying agent in culture media D. Colony – a population of microbial cells arising from a single cell or spore or from a group of attached cells E. Capnophile – microbe requiring an elevated molecular oxygen (O2) environment for optimal growth
4. (a) Which of the following chemical reactions is an overall oxidation-reduction (redox) reaction that involves oxidation number changes in their reactions? A. NaOH + H₂O → Na¹ + H3O+ + OH B. HCl + H₂O → H3O+ + Cl- C. CO₂ + H₂O → H+ + CO3²- D. N₂ + 3H₂ → 2NH3
4. Which ranges of microbial colony count is ideal standard for a good 1, plate in Pour Plate technique? Check all that apply. * O TNTC (too numerous to count) O 25-250 colonies O 10-1000 colonies O 30-300 colonies 5. How many colony-forming units (CFUS) of bacteria are there in a gram of soil if 120 colonies grow in a nutrient agar (NA) plate with 1 ml inoculum from 1:10,000 dilution? * 120 O 1,200 120.000 1,200,000 6. How much volume of microbial inoculum will be dispensed into a sterile nutrient agar plate in Spread Plate technique of bacterial isolation? 0.1 ml 1 ml 10 ml O 100 ml 7. Which materials are to be prepared when using Spread Plate technique for bacterial isolation from water sample of oligotrophic Lake Lanao? Check all that apply. * O lake water sample O sterile nutrient agar plate O sterilizable glass rod O flame source
4) You are interested in the total bacterial load of the bat guano. In order to determine this, you go back to your original broth culture and prepare a dilution series. You then spread 100 ml of each dilution onto 3 separate plates. You obtain the following results: Plate 1, 10 -1 dilution: 362 colonies Plate 2, 10 -3 dilution: 76 colonies Plate 3, 10 -5 dilution: 8 colonies. Based on your results, calculate the CFUs/ml in your original broth culture. show your work
Answer and the image below and provide explanations.
SPREAD PLATE See the follow up procedure for determining colony counts and express the number of colony forming units (CFU) in scientific notation. Record "TNTC" for plates with colonies that are too numerous to count. CFU/mL-Use scientific notation DILUTION 1:107 1:108 COLONY COUNT ...دما
Calculate the doubling time of a culture of E. coli that is started with 3x105 CFU/mL and grows to 4.6x108 CFU/mL in 3 hours. Be sure to use units in your answer.
N. benthamiana will be infiltrated with a solution containing OD600=0.3 of each experimental Agro containing construct and OD600=0.1 of p19. Calculate the volume of cultures (V construct; V p19) needed according to the formulas: V construct = V final × 0.3/OD600; V p19 = V final × 0.1/OD600. One mL of infiltrate is often enough to complete a small experiment. How to plan your final volume accordingly?
Please give explanation for each step other give dislike
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The following is result from a standard plate count. What is the cell density of the undiluted culture? Please follow all SPC rules. (TNTC: too numerous to count)
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