A bacterial strain is growing exponentially. At 1:00pm the titre of the culture is 2x10^3 cells per ml. Three hours later, the culture is still growing exponentially and the titre is 4x10^4 cells per ml. How many generations have elapsed, what is the generation time, and what will the titre be at 9:00pm

Q: The solution containing bacterial spores is heated in an autoclave. When the autoclave has reached a…

A: Given that          Number of spores after autoclave reaches 121 C (No) = 106 spores/ml…

Q: If a culture had 4 cells to begin with and has a generation time of 60 minutes, how long would it…

A: "Microbiology" is the study of microorganisms and associated topics. Microbiology has gone a long…

Q: A culture with approximately 3x104 cells/mL were incubated. After 3 hours, the number of cells had…

A: The number of generations (n) refers to the number of times a cell population got doubled during a…

Q: In a plate count, 1 mL of culture is spread on a plate and incubated for 24 hours. 250 colonies are…

A: Given,number of colonies in a 1mL of culture = 250. Also,given that this given solution is 100 times…

Q: Consider the growth curve shown below. Cells were grown in a medium containing 1 % w/v glucose and…

A: The given graph represent the biphasic growth of cells. Cells are grown on two carbon sources…

Q: You have a culture that has 5 X103 cfu/ml of bacteria in it. You perform a serial dilution by adding…

A: In microbiology and bacteriology, the technique of serial dilution is a fundamental method used to…

Q: A bacteria colony's radius is measured as a function of time. t r(t) 0 1.01 0.1 1.031 Estimate the…

A: In microbiology, the growth rate of a bacterial colony is an important parameter that helps us…

Q: Use the information in the tables below to determine the most probable number of bacteria per 100 mL…

A: The Most Probable Number (MPN) method, which replicates liquid broth growth in ten-fold dilutions,…

Q: Which cultures would be suitable for the transduction experiment and why？ Which cultures would not…

A: Transduction is a process of gene transfer in bacteria, where bacterial DNA is transferred from one…

Q: Assume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If…

A: Fibronectin is a glycoprotein found in the extracellular matrix of cells and is also obtained from…

Q: The PD# of your culture is 165. You plated 3745 cells per well. You have recovered 12,655 cells.…

A: The passage number of a cell culture is a record of the number of times the culture has been…

Q: The lag phase does NOT occur if an exponentially growing culture is transferred into a nutrient rich…

A: Microbial growth is said to occur when there is an increase in the number of cells rather than an…

Q: Three different strains of Escherichia coli, respectively A, B, and C have been inoculated in three…

A: E.coli cells take around 20 mins to replicate themselves. The replication of bacteria is an…

Q: Below are the results of streak for isolations of Bacterium 1 and Bacterium 2 before and after they…

A: Pure culture :- Pure culture is one that has only one kind of bacterial population which earlier…

Q: You want to create a 10-fold dilution . You have a saline tube with a volume of 450ul. How much of…

A: According to this theory, a diluted solution's volume will be 10 times greater than its stock…

Q: Dilute cells from an old culture 1:200 into 5 mL of fresh media. What volume of the old culture…

A: Introduction Dilution is the process of lowering the concentration of a solute in a solution by…

Q: Below is a set of data for a serial dilution/plate count experiment.    Based on this data what is…

A: There are numerous methods/techniques for counting the number of bacteria in a particular sample. A…

Q: A culture of bacteria is found to have a DRT of 2 minutes. How long would it take to reduce the…

A: Heat is used for sterilization of those substances that can withstand it over longer periods or…

Q: Refer to the provided image drawn by a student trying to plan out their serial dilution protocol.…

A: Colony forming unit (CFU)is a measure of viable bacterial cell. CFU/ml can be calculated using…

Q: A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution…

A: A sample of your blood, urine, skin, or any part of your body will be taken during a bacteria…

Q: If the 10-9 plate was counted to have 155 colonies on it, would the CFU/mL be 1.55*1011? If not,…

A: CFU  Colony forming unit it a method to calculate the number of microorganisms in a culture media.

Q: What method of bacterial measurement does this graph represent? Is it considered direct, or…

A: Here Bacterial growth is measured in terms of absorbance so, bacterial growth is measured with the…

Q: You have several different media onto which you inoculated eight strains of yeast (A-H). The media…

A: Minimal medium (unsupplemented) A minimal medium is a growth medium used for growing microbes and…

Q: A slant is inoculated with 100 E.coli cells. The slant is left at room temperature where the…

A: Bacterial cells divide by binary fission to produce two daughter cells. The growth of a bacterial…

Q: A 10-2 is performed on a culture of bacteria in order to perform viable plate counts. From the…

A: CFU means colony forming unit. It is a measure of viable bacterial or fungal cells. In case of…

Q: Draw a semi-logarithmic diagram showing the killing kinetics of a vegetative bacterial culture,…

A: Because they can inactivate vegetative cells, bacterial spores, yeast, and moulds, thermal…

Q: (b) In a batch bioreactor, the growth of a microbial population is often limited by the availability…

A: As the question contain two different questions, here we have only solved the second one. Bacteria…

Q: To determine the number of cells in a pure culture of Klebsiella pneumoniae, you have performed a…

A: Colony-forming units per millilitre (cfu/ml) of Klebsiella pneumonia original culture can be found…

Q: Compare and contrast bacteriocidal, bacteriostatic and bacteriolytic agents. What are their effects…

A: It is critical that we understand microbes and their applications since they may be both useful and…

Q: 6.0 EXPECTED RESULT FOR BACTERIAL GROWTH CURVE Table 1 Culture 1,0ptical Density (OD 550) 0.07 0.07…

A: The growth curve is made between OD versus incubation time.

Q: Define what is a minimal medium and the nature of its components.

A: A culture medium can be defined as solid,liquid or semi solid medium which helps in support of the…

Q: what is the decimal reduction time?

A: D-value or the Decimal reduction rate is the required time to achieve 90% killing/ reduction of…

Q: A total of 2.5 x 105 cells were harvested from the patient. You need the cells to fill a scaffold…

A: For calculation of volume we need to know the basic formula of l×b×h

Q: Which of the answers below represents the correct mathematical expression of bacterial growth of…

A: The formula for bacterial growth is as follows: Nt = No × 2n Where Nt= bacterial population at time…

Q: How would you produce a 10^-1 dilution if a 3 mL bacterial sample using the entire 3 mL volume?…

A: Dilution factor refers to the ratio of the volume of the initial (concentrated) solution to the…

Q: What would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x…

A: The dilution of a bacterial culture allows viable cell count when plated on the solid media. Serial…

Q: You are growing a culture of S. aureus. You know that 9 generations have occurred after 6 hours.…

A: Introduction  Binary fission, a process that causes bacteria to divide into two daughter cells, is…

Q: A total of 2.5 x 105 cells were harvested from the patient. You need the cells to fill a scaffold…

A: The growth or culturing of cells obtained from different sources such as humans, animals, or plants…

Q: D) Comment on the growth curve, is the bacteria growing exponential?

A:

Q: 109 108 Wild Type tlc1 107 - tlc1 rad59 106 - tlc1 rad50 rad59 - tlc1 rad51 rad59 105 • tlc1 rad50…

A: The RAD 59 is a gene present in the yeast strain Saccharomyces cerevisiae. The proteins encoded by…

Population Growth

R and K Selection

R and K selection are concepts in ecology used to describe traits in the fluctuation of a population or population dynamics. For example, they describe the life-association traits between parent and offspring, such as quantity or number of young ones born at a time, quality of parental care, the age to maturity, and reproductive effort.