1. DNA replication is described as semi-conservative becauseA. one leading strand and one lagging strand are produced in DNA replicationB. all new DNA strands are synthesized continuouslyC. one new DNA strand is synthesized discontinuouslyD. DNA replication can never produce DNA molecules which consist of both original DNAstandsE. one DNA strand is the template while the complementary strand is not2. Processivity of a polymerase refers toA. whether the polymerase can process the 5'end of the synthesized moleculeB. how long a poly-A tail can be formedC. the efficiency of the removal of intronsD. the efficiency in editingE. how long a new strand can be synthesized without dissociation3. Which of the following processes does NOT hydrolyse ATP or GTP?A. Rho-dependent termination of DNA replicationB. Unwinding DNA by helicaseC. Complementary base-pairing between A and TD. Formation of fMet-tRNAE. Translocation of ribosome by EF-G4. An RNA produced from a fragment of DNA has the sequence of 5'AAUUGGCU3'. Thesequence of the nontemplate strand in the DNA that gives rise to this sequence should beAll sequences below are written in 5'→3'.A. AGCCAATTB. AAUUGGCUC. AATTGGCTD. TTAACCGAE. UUAACCGA

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Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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A mistake in DNA replication, in which the wrong base is placed in the growing DNA strand, can have profound effects on the descendents of any cells that carry the mistake.In the following strand, the A has been changed to a G. Draw the DNA molecules that result from replication of the altered strands. Original Strand T A G C G T T Mutated Strand T G G C G T T A T C G C A A A T C G C A A
Below is a study of a colony of cells, determine that some of these cells have a mutated DNA polymerase I that results in loss of function of this enzyme. - What will the effect of the mutation in DNA polymerase I be on DNA replication? Include leading and lagging strand - Will this mutation in DNA polymerase I have an impact on another step in DNA replication? Will DNA be replicatation be impacted? Are any enzymes involved?
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1. A.) Deletion mutation is a loss of a single base by damage. B.) Point mutation is when a nucleotide is paired with a wrong nucleotide during RNA replication. a. Statement A is correct b. Statement B is correct c. Both A and B are correct d. Both A and B are incorrect Answer: 2. This is a process that produces a change in the structure of the DNA by forming adenine rather than guanine. a. Reverse transcription of guanine b. Alkylation of the 06-Guanine c. Reverse translation of guanine d. Methylation of guanine bases Answer: 3. This type of DNA repair happens when you are removing a single damaged base of DNA strand. a. Nucleotide repair b. Base excision repair c. Strand break repair d. Base nucleotide replacement Answer:
1. Identify the three protein complexes/enzymes important in eukaryotic DNA replication A. Enhancer, silencer, promoter B. DNA polymerase, RNA polymerase, mediator C. Start codon, stop codon, RNA polymerase D. Prereplication complex, origin recognition complex, MCM helicase
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5.A. Draw a wild-type replication fork showing leading and lagging strands and their 5 to 3' direction of synthesis (arrow). B. Draw a replication fork in a RNA primase mutant (no RNA primase function). C. Draw a replication fork in a DNA helicase mutant. D. Draw a replication fork in a DNA polymerase mutant that lacks the proofreading function (but possesses all the other DNA polymerase functions).
3) Why are single strand binding proteins (ssb proteins) needed in DNA replication? (note that you need to explain WHY ssb proteins are important, not just what they do)
5. Draw the major components of the DNA Pol III replication complex in the context of a replication fork. Be sure to illustrate and label the leading and lagging strands, RNA primers, Okazaki fragments, the polymerase core, the beta-clamp subunits, and the clamp loader complex, and show how the lagging strand is threaded through the replication complex.
RNA primers are necessary in DNA synthesis because OA. primers mark the sites where helicase has to unwind the DNA. OB. the DNA polymerase enzyme can catalyze the addition of deoxyribonucleotides only onto the 3' (-OH) end of an existing strand. O C. RNA primase is the first enzyme in the replication complex. D. they are the source of nucleotides for DNA polymerases. 00 O E. DNA polymerase is unable to initiate replication without an origin. 5 p
At each origin of replication, DNA synthesis proceeds bidirectionally from two replication forks. Which of the following would happen if a eukaryotic mutant arose having only one functional fork per replication bubble? (See diagram.)a. No change at all in replication. b. Replication would take place only on one half of the chromosome. c. Replication would be complete only on the leading strand. d. Replication would take twice as long.
The diagram depicts one of the two strands of DNA, at the end of a chromosome (top strand). Replication of this strand produced the two fragments shown at the bottom of the diagram. Which of the statements below is correct regarding filling the gaps that exist in the newly synthesized DNA? Hint: remember the directionality requirement for DNA polymerase. 5' 0 0 0 0 (a) gap (a) can be filled gap (b) can be filled both gaps (a) and (b) can be filled neither gaps (a) nor (b) can be filled (b) 3'

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