1. Cytosine deamination, in which cytosine is mutated to uracil, occurs spontaneously in DNA at a rate of 1 out of107 cytosines in 24 hours.tofoNH₂'Ncytosine deamination----ဝိNHspra. If cytosine deamination occurs and DNA polymerase replicates the DNA with this altered base, whatmutation would be introduced into DNA?b. Cytosine deamination is so common that DNA repair enzymes recognize uracil and replace it withcytosine. Given this information, why is it essential for DNA, the carrier of the genetic blueprint, toutilize thymine, not uracil, as a base?

Nitrogenous bases are cyclic compounds that contain nitrogen. They can be: Purines are heterocyclic…

Answered: 1. Cytosine deamination, in which…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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9 form a covalent linkage to both strands of the DNA helix at the same time, making a translent double-stranded break in the helix. These enzymes are activated by sites on a chromosome where two double helices cross over each other. Which one. DNA gyrase DNA helicase Topoisomerase I Single- strand DNA-binding (SSB) protein DNA polymerase Topoisomerase II
33.Choose the DNA nucleotide sequence that would be complementary to the following strand (note: pay attention to DIRECTION of replication): 5' GTAGTCA 3' a 3' UATUAGA 5' b. 5' TGACTAC 3' 3' ACGACTG 5' 5' UGACUAC 3'. 5.Using agarose gel electrophoresis to analyze DNA, short DNA travels the long DNA. a faster than slower than at the same speed as d. Question 6 Which type of bonds link the individual nucleotides together in DNA? Glycosidic bond Peptide bond Phosphodiester bond Electrostatic bond a e Hydrogen bond Question 7 A typical engineered plasmid vector often contains the following features except a A poly linker region Origin of replication A signal peptide An antibiotic resistant gene a. b. C. C. d.
8-oxo-G base in genomic DNA is often produced by_ and may be repaired by Select one: O a. reactive oxygen species-----MutM O b. UV radiation-------MutS O c. UV radiation-------MutM O d. reactive oxygen species------MutS
9. H. State if/how Aureliano's mutation changes the amino acid sequence and describe the effect that change has, if any, on the protein.
6. H₂C HO O O T "N Which of the following dinucleotides can be methylated by DNA methyltransferase? NH₂ NH₂ N N NH NH C N NH N NH "N topol NH₂ Dofo HO G 01-07 HO "NH₂ NH₂ N NH, QP-C NH₂ Joy HO HO НО B C D 7. Explain the effect of the acetylation status of histones on chromatin structure and gene expression. Your answer should include the terms "heterochromatin", "euchromatin", "transcriptionally active" and "transcriptionally inactive" Dofo HO
5. DNA is made of two strands that are antiparallel. If one strand runs from 3' to 5' direction the other one will go from 5' to 3' direction. During replication or transcription, whatever the process is, it will always follow the 5' to 3' direction using the 3' to 5' directed strand as the template strand. Therefore, if following is the DNA sequence 5'-CCG ATC GCA CAA-3' a) Using this sequence as template after transcription no protein can be translated. Why? I. Presence of start codon II. Absence of start codon III. Due to mutation b) If you want to start the translation, what change you need in the second codon (from 5' to 3' direction)? I. Substitution of C with G II. No change III. Deletion of C IV. Both I & III
13. Draw deoxyribonucleosides in the conformation they would appear in Z-DNA and B-DNA.
47 If base #6 is changed from A to T, what type of mutation is this?? ANTISENSE STRAND 5' ATTTGACGG 3' Second letter UUU), Phe UCU UAU1. UGU UUC UCC UAC Tyr UGC Cys Ser UUA UCA UAA Stop UGA Stop UAG Stop UGG Trp Leu UUG UCG CU CCU CUC Leu CUA CC CCA CG CAU) His CAC. Pro CAA Gin CAG CGU CGC Arg CUG CGA CGG AAU AUU AUC le AUA AUG Met ACG ACU ACC AGU AAC Asn AGC Ser Thr ACA AAA) AGA AAG Lys AGG Arg GCU GCC GCA Ala GUU GAU GGU GUC Val GACJASP GGC G GAAT GGA Gly GUA Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an ansWer. a silent frameshift nonsense missense Third letter First letter
10
13
12. Shown below are three possible mechanisms for DNA replication. Identify which each kind is (semiconservative, conservative, or dispersive). I Which model is this? (select one) A. Semiconservative Conservative Dispersive Semiconservative Conservative Dispersive Semiconservative Conservative Dispersive B. C. XXX< XXXXXX XXX< x< XXXXXXXX XXXXXXXxXxXx XXXXXX MXXXXX XXxxxxxX XXXXXX
€ 2 A X 1_30*_SP23 - General Biology I (for majors)/11 f us page The anticodon sequence created from the following DNA: TACGGGGCTGAGATT Select one: a. AUGCCCCGACUCUAA O b. Met-Pro-Arg-Leu-STOP O c. UACGGGGCUGAGAUU O d. Tyr-Gly-Ala-Glu-lle 20 000 MacBook Air DII

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