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You perform the following solid-phase oligonucleotide synthesis: DMT lodine, water, pyridine DMT-O acetic anhydride, trichioroacetic acid N-methylimidazole tetrazole DMT

You perform the following solid-phase oligonucleotide synthesis: DMT lodine, water, pyridine DMT-O acetic anhydride, trichioroacetic acid N-methylimidazole tetrazole DMT

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question 4 You perform the following solid-phase oligonucleotide synthesis: NH DMT DMT-O lodine, water, acetic anhydride, N-methylimidazole trichloroacetic acid tetrazole pyridine HN DMT lodine, water, acetic anhydride, N-methylimidazole aq, ammonia trichloroacetic acid tetrazole pyridine Which product do you expect? O 5'-TAG-3' O 5'-CGA-3' O 5'-GAT-3' O 5'-AGT-3' O 5'-TGA-3' O 5'-AGC-3'
Transcribed Image Text:Question 4 You perform the following solid-phase oligonucleotide synthesis: NH DMT DMT-O lodine, water, acetic anhydride, N-methylimidazole trichloroacetic acid tetrazole pyridine HN DMT lodine, water, acetic anhydride, N-methylimidazole aq, ammonia trichloroacetic acid tetrazole pyridine Which product do you expect? O 5'-TAG-3' O 5'-CGA-3' O 5'-GAT-3' O 5'-AGT-3' O 5'-TGA-3' O 5'-AGC-3'
Question 6 What is wrong about the following depiction of DNA polymerase in action? Select all things that are wrong -O- "O-P=0 ОН OH HO ОН NH2 H2N NH2 NH2 NH2 OH OH OH The DNA contains a uracil base, but DNA should have thymine in stead The base being incorporated is not complementary to the base in the template | This is actually RNA polymerase producing RNA | The extender unit used by DNA polymerase should be a nucleoside diphosphate, not a triphosphate | The mechanism depicted does not match the reaction catalyzed by DNA polymerase
Transcribed Image Text:Question 6 What is wrong about the following depiction of DNA polymerase in action? Select all things that are wrong -O- "O-P=0 ОН OH HO ОН NH2 H2N NH2 NH2 NH2 OH OH OH The DNA contains a uracil base, but DNA should have thymine in stead The base being incorporated is not complementary to the base in the template | This is actually RNA polymerase producing RNA | The extender unit used by DNA polymerase should be a nucleoside diphosphate, not a triphosphate | The mechanism depicted does not match the reaction catalyzed by DNA polymerase
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