1) Determine how many mg of your compound you will need to make your 100 mM stock solution. Your final volume should be 250 μL. 2 2) Determine how many μL of 100 mM stock and how many μL of DMSO you will need to add to make your 10 mM stock. You should use M,V₁ = MV to perform this calculation where M₁ = 100 mM, M₁ = 10 mM, V, is 250 μL, and V, is what you are solving for. Remember, V, represents your total final volume. You can determine how much DMSO you need to add by subtracting V, from 250 μL. 1 2 3) Determine how many µL of 10 mM stock and how many μL of DMSO you will need to add to make your 1 mM stock. You should set up your calculations similar to the way you set them up in step 2. 4) Determine how many μL of 1 mM stock and how many μL of DMSO you will need to add to make your 0.1 mM stock. You should set up your calculations similar to the way you set them up in step 2. 5) On the day of the experiment, you will take 10 μL of your 1 mM stock and place it in a well with a total final volume of 200 μL. What will be the final concentration of compound in the well? You should set up your calculations similar to the way you set them up in step 2. 6) On the day of the experiment, you will take 10 µL of your 0.1 mM stock and place it in a well with a total final volume of 200 μL. What will be the final concentration of compound in the well? You should set up your calculations similar to the way you set them up in step 2. 7) On the day of the experiment, you will take 10 μL of your 0.01 mM stock and place it in a well with a total final volume of 200 μL. What will be the final concentration of compound in the well? You should set up your calculations similar to the way you set them up in step 2.
1) Determine how many mg of your compound you will need to make your 100 mM stock solution. Your final volume should be 250 μL. 2 2) Determine how many μL of 100 mM stock and how many μL of DMSO you will need to add to make your 10 mM stock. You should use M,V₁ = MV to perform this calculation where M₁ = 100 mM, M₁ = 10 mM, V, is 250 μL, and V, is what you are solving for. Remember, V, represents your total final volume. You can determine how much DMSO you need to add by subtracting V, from 250 μL. 1 2 3) Determine how many µL of 10 mM stock and how many μL of DMSO you will need to add to make your 1 mM stock. You should set up your calculations similar to the way you set them up in step 2. 4) Determine how many μL of 1 mM stock and how many μL of DMSO you will need to add to make your 0.1 mM stock. You should set up your calculations similar to the way you set them up in step 2. 5) On the day of the experiment, you will take 10 μL of your 1 mM stock and place it in a well with a total final volume of 200 μL. What will be the final concentration of compound in the well? You should set up your calculations similar to the way you set them up in step 2. 6) On the day of the experiment, you will take 10 µL of your 0.1 mM stock and place it in a well with a total final volume of 200 μL. What will be the final concentration of compound in the well? You should set up your calculations similar to the way you set them up in step 2. 7) On the day of the experiment, you will take 10 μL of your 0.01 mM stock and place it in a well with a total final volume of 200 μL. What will be the final concentration of compound in the well? You should set up your calculations similar to the way you set them up in step 2.
Chemistry: The Molecular Science
5th Edition
ISBN:9781285199047
Author:John W. Moore, Conrad L. Stanitski
Publisher:John W. Moore, Conrad L. Stanitski
Chapter3: Chemical Reactions
Section: Chapter Questions
Problem 87QRT: You need 300. mL of 0.500-M K2Cr2O7. Which method is best to prepare this solution? Explain your...
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Give an example and show how to do calculations
Transcribed Image Text:1) Determine how many mg of your compound you will need
to make your 100 mM stock solution. Your final volume
should be 250 μL.
2
2) Determine how many μL of 100 mM stock and how many
μL of DMSO you will need to add to make your 10 mM
stock. You should use M,V₁ = MV to perform this
calculation where M₁ = 100 mM, M₁ = 10 mM, V, is 250
μL, and V, is what you are solving for. Remember, V,
represents your total final volume. You can determine how
much DMSO you need to add by subtracting V, from 250
μL.
1
2
3) Determine how many µL of 10 mM stock and how many
μL of DMSO you will need to add to make your 1 mM
stock. You should set up your calculations similar to the
way you set them up in step 2.
4) Determine how many μL of 1 mM stock and how many μL
of DMSO you will need to add to make your 0.1 mM stock.
You should set up your calculations similar to the way you
set them up in step 2.
5) On the day of the experiment, you will take 10 μL of your
1 mM stock and place it in a well with a total final volume
of 200 μL. What will be the final concentration of
compound in the well? You should set up your calculations
similar to the way you set them up in step 2.
6) On the day of the experiment, you will take 10 µL of your
0.1 mM stock and place it in a well with a total final
volume of 200 μL. What will be the final concentration of
compound in the well? You should set up your calculations
similar to the way you set them up in step 2.
7) On the day of the experiment, you will take 10 μL of your
0.01 mM stock and place it in a well with a total final
volume of 200 μL. What will be the final concentration of
compound in the well? You should set up your calculations
similar to the way you set them up in step 2.
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