BIOS255 Lab Week7

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Chamberlain University College of Nursing *

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255

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Mechanical Engineering

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Feb 20, 2024

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Paola Rodriguez BIOS255 Week 7 Respiratory System-Physiology Learning objectives:  Explain physiologic adaptations of the cardiorespiratory system of seals to deep diving.  Point out differences between seal and human physiology related to deep dives.  Evaluate respiratory and cardiac function.  Measure oxygen consumption and calculate the total amount of oxygen needed for dives of various durations and compare to estimated stores in the lungs, blood and tissues. Introduction: Active cells require a constant supply of oxygen in order to produce energy to carry out cellular processes and release carbon dioxide as a waste product. The body must replenish this supply of oxygen and remove the build-up of carbon dioxide, as it is toxic to cells in high concentrations. Our respiratory rate, or the number of breaths we take per minute, and the depth at which we respire with each breath is controlled by the respiratory center, located in the brainstem. It ensures that our respiratory effort matches the metabolic demands of our body. In this lab you will head to a research lab in Antarctica to learn how seals have adapted to perform long, deep dives. How Weddell seals can dive up to 600 meters for 30 minutes is a bit of a mystery. In this simulation you will learn about oxygen stores, aerobic dive limit, aerobic and partially anaerobic ATP generation, and special cardio- respiratory adaptations that allow seals to perform deep, long dives that would be impossible for humans without scuba diving equipment. This will be a fun way to learn more about human cardiorespiratory physiology as well. Humans and seals have different amounts of oxygen available to them and store it at different proportions in lungs, blood, and muscle. Do seals and humans have the same factorial increase in oxygen consumption? Monitor 3 dives and collect data on oxygen and lactate to find out! Assignment: Part 1: Complete Labster “Cardio-respiratory Physiology: How can seals dive so deep for so long?” As you complete the lab, have the lab report ready to record data. The theory section of the lab is a useful resource. Part 2: Complete the lab report. Respiratory Physiology Lab Report

1. Compare the diving depths and oxygen stores and aerobic dive limits : (5 points) Weddell seal Human Diving depth 600 meters 40 meters mL O 2 in lungs 1200 900 mL O 2 in blood 21950 1005 mL O 2 in muscle 9005 188 Total mL O 2 in stores before dive 32155 2093 % of Oxygen in blood 71% 59% % of Oxygen in muscle 25% 16% % of Oxygen in lungs 4% 25% Aerobic dive limit (mins) Predicted if have the same factorial increase in oxygen usage as humans: 10.2 Actual: 23.8 1.4

2. a. What is the main difference between seals and humans regarding oxygen stores? (1 point) Seals store approximately three times the amount of oxygen per kilogram of body mass compared to humans. They store more oxygen in the blood and less in the lungs compared to humans. b. Where is the greatest proportion of oxygen stored in humans? (1 point) In the blood 3. Do seals show approximately the same factorial increase in oxygen consumption as humans during diving? (1 point) Seals demonstrate a significantly lower rate of increase in oxygen consumption than humans, approximately 10 minutes compared to 1:24 minutes for humans. 4. How did the rate of oxygen consumption during diving change between a seal diving for 12 mins vs diving for 30 mins? (1 point) 5. Seals used approximately the same or a 6. little less O2 per min for the 30 min dive 7. Seals used approximately the same or a

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8. little less O2 per min for the 30 min dive Seals used approximately the same or a little less O2 per min for the 30 minute dive. 5. Explain why some lactate accumulated in the 30 min dive vs none in the 12 min dive. (2 points) It indicated that seals are using anaerobic metabolism when they dive. 6. How does the seal’s heart respond to diving? (1 point) It decreases. 7. What do the heart rate and consumption rate patterns suggest about seal diving? (1 point) The suppression of heart rate may help reduce the energy cost of diving. 8. You’ve learned so much about the physiology of diving, and major adaptations of seals that enhance their oxygen storage capacity and ability to dive. Which of the following is an INCORRECT statement regarding seal adaptations? (1 point) a. Seals have larger lungs than humans per body mass b. Seals have a higher content of hemoglobin and larger blood volume than humans c. Seals have a higher content of myoglobin in the muscle relative to humans. d. Seals have smaller lungs than humans per body mass. 9. How does the respiratory system complement the circulatory system? (1 point) The respiratory system is responsible for supplying the body with oxygen in addition to its circulatory system. Oxygen-rich blood is circulated through the bloodstream through the respiratory system.

Grading Rubric for Lab Report Activity Deliverable Points Part 1 Complete Labster 15 Part 2 Complete lab report 15 Total Complete all lab activities 30